Protein Info for HMPREF1078_RS17155 in Parabacteroides merdae CL09T00C40
Annotation: gluconate 5-dehydrogenase
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
Swiss-Prot: 39% identical to FABG_THEMA: 3-oxoacyl-[acyl-carrier-protein] reductase FabG (fabG) from Thermotoga maritima (strain ATCC 43589 / MSB8 / DSM 3109 / JCM 10099)
KEGG orthology group: K00046, gluconate 5-dehydrogenase [EC: 1.1.1.69] (inferred from 95% identity to bsa:Bacsa_2429)MetaCyc: 84% identical to 2-dehydro-3-deoxy-D-gluconate 5-dehydrogenase monomer (Streptococcus agalactiae NEM316)
2-dehydro-3-deoxy-D-gluconate 5-dehydrogenase. [EC: 1.1.1.127]
Predicted SEED Role
"2-deoxy-D-gluconate 3-dehydrogenase (EC 1.1.1.125)" in subsystem D-Galacturonate and D-Glucuronate Utilization (EC 1.1.1.125)
MetaCyc Pathways
- 4-deoxy-L-threo-hex-4-enopyranuronate degradation (5/5 steps found)
- 2-deoxy-D-glucose 6-phosphate degradation (2/4 steps found)
- 3,6-anhydro-α-L-galactopyranose degradation (4/7 steps found)
- L-idonate degradation (1/3 steps found)
- ketogluconate metabolism (1/8 steps found)
- superpathway of microbial D-galacturonate and D-glucuronate degradation (15/31 steps found)
KEGG Metabolic Maps
Isozymes
Compare fitness of predicted isozymes for: 1.1.1.125
Use Curated BLAST to search for 1.1.1.125 or 1.1.1.127 or 1.1.1.69
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
Find the best match in UniProt
Protein Sequence (263 amino acids)
>HMPREF1078_RS17155 gluconate 5-dehydrogenase (Parabacteroides merdae CL09T00C40) MVNFSLEGKVALVTGASYGIGFAIATAFAKAGATIVFNDIKQELVDKGLAAYEAEGIKAH GYVCDVTNEEQVNAFVAQVEKEVGVIDILVNNAGIIKRIPMVEMTAAEFRQVIDIDLNGP FIVSKAVIPSMIKKGHGKIINICSMMSELGRETVSAYAAAKGGLKMLTRNIASEYGEYNI QCNGIGPGYIATPQTAPLRERQADGSRHPFDAFIVAKTPAARWGTPEDLMGPAVFLASDA SNFVNGHVLYVDGGILAYIGKQP