Protein Info for HMPREF1078_RS17110 in Parabacteroides merdae CL09T00C40

Annotation: tRNA pseudouridine(55) synthase TruB

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR00431: tRNA pseudouridine(55) synthase
  • amino acids 8 to 216 (209 residues), 209.1 bits, E=3.2e-66
• PF01509: TruB_N
  • amino acids 34 to 181 (148 residues), 185 bits, E=1.1e-58
• PF16198: TruB_C_2
  • amino acids 182 to 222 (41 residues), 30.7 bits, 2.8e-11

Best Hits

Swiss-Prot: 89% identical to TRUB_PARD8: tRNA pseudouridine synthase B (truB) from Parabacteroides distasonis (strain ATCC 8503 / DSM 20701 / CIP 104284 / JCM 5825 / NCTC 11152)

KEGG orthology group: K03177, tRNA pseudouridine synthase B [EC: 5.4.99.12] (inferred from 89% identity to pdi:BDI_2406)

Predicted SEED Role

"tRNA pseudouridine synthase B (EC 4.2.1.70)" in subsystem tRNA processing (EC 4.2.1.70)

MetaCyc Pathways

• pseudouridine degradation (1/2 steps found)

KEGG Metabolic Maps

• Pyrimidine metabolism

Isozymes

Compare fitness of predicted isozymes for: 4.2.1.70, 5.4.99.12

Use Curated BLAST to search for 4.2.1.70 or 5.4.99.12

Protein Sequence (236 amino acids)

>HMPREF1078_RS17110 tRNA pseudouridine(55) synthase TruB (Parabacteroides merdae CL09T00C40)
MDFIAGEVLYFNKPLKWTSFDLVNKFRYKLSRKLKVKKIKVGHAGTLDPLATGVMIVCTG
KATKRIDEFQYQTKEYVATLKLGETTPSFDLEKEIDGVYPTEHITREEVEKVLQSFVGTI
QQIPPVFSACKVDGKRAYELARKGEEVELKSKTLVIDEMELLECDLPVIKIRVVCSKGTY
IRALARDIGVALQSGAHLIALERTRIGDITLAKCMSPDDIDTFLDENIIIRKDCED