Protein Info for HMPREF1078_RS16675 in Parabacteroides merdae CL09T00C40

Annotation: ketol-acid reductoisomerase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF07991: IlvN
  • amino acids 27 to 192 (166 residues), 143.2 bits, E=8.5e-46
• TIGR00465: ketol-acid reductoisomerase
  • amino acids 28 to 346 (319 residues), 309.6 bits, E=1e-96
• PF03446: NAD_binding_2
  • amino acids 33 to 116 (84 residues), 21.5 bits, E=3.1e-08
• PF01450: IlvC
  • amino acids 200 to 344 (145 residues), 138.2 bits, E=3.7e-44

Best Hits

Swiss-Prot: 68% identical to ILVC_DESOH: Ketol-acid reductoisomerase (NAD(+)) (Dole_2039) from Desulfococcus oleovorans (strain DSM 6200 / Hxd3)

KEGG orthology group: K00053, ketol-acid reductoisomerase [EC: 1.1.1.86] (inferred from 86% identity to bsa:Bacsa_0835)

Predicted SEED Role

"Ketol-acid reductoisomerase (EC 1.1.1.86)" in subsystem Branched-Chain Amino Acid Biosynthesis or Coenzyme A Biosynthesis (EC 1.1.1.86)

MetaCyc Pathways

• superpathway of branched chain amino acid biosynthesis (16/17 steps found)
• superpathway of coenzyme A biosynthesis I (bacteria) (9/9 steps found)
• superpathway of L-isoleucine biosynthesis I (11/13 steps found)
• L-valine biosynthesis (4/4 steps found)
• phosphopantothenate biosynthesis I (4/4 steps found)
• superpathway of L-threonine metabolism (14/18 steps found)
• L-isoleucine biosynthesis I (from threonine) (6/7 steps found)
• pyruvate fermentation to isobutanol (engineered) (3/5 steps found)
• phosphopantothenate biosynthesis III (archaea) (2/4 steps found)
• L-isoleucine biosynthesis III (4/7 steps found)
• superpathway of coenzyme A biosynthesis II (plants) (4/10 steps found)

KEGG Metabolic Maps

• Biosynthesis of alkaloids derived from histidine and purine
• Pantothenate and CoA biosynthesis
• Valine, leucine and isoleucine biosynthesis

Isozymes

Compare fitness of predicted isozymes for: 1.1.1.86

Use Curated BLAST to search for 1.1.1.86

Protein Sequence (347 amino acids)

>HMPREF1078_RS16675 ketol-acid reductoisomerase (Parabacteroides merdae CL09T00C40)
MAVMNFGGVDENVVTREEFPLEKAREVLKDEVIAVIGYGVQGPGQSLNLRDNGFNVIVGQ
RPGKTYDKAVADGWVPGETLFGIEEACERATIIQVLLSDAAQIECWPRIKKYLTPGKALY
FSHGFAITYKERTNIIPPADVDVILVAPKGSGTSLRRMFLQGRGLNSSYAIFQDATGRAW
DRVIALGIGVGSGYLFETTFKKEVYSDLTGERGTLMGAIQGLLLAQYETLRENGHEPSEA
FNETVEELTQSLMPLFAENGMDWMYANCSTTAQRGALDWMGPFHDAVKPVFEKLYREVAC
GNEAQRSIDTNSKPDYREGLEKELAALRESEMWRAGAVVRKLRPENN