Protein Info for HMPREF1078_RS16665 in Parabacteroides merdae CL09T00C40

Annotation: 2-isopropylmalate synthase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 498 TIGR00973: 2-isopropylmalate synthase" amino acids 4 to 494 (491 residues), 659.1 bits, see alignment E=1.8e-202 PF00682: HMGL-like" amino acids 6 to 272 (267 residues), 305.9 bits, see alignment E=2.6e-95 PF08502: LeuA_dimer" amino acids 369 to 496 (128 residues), 111.5 bits, see alignment E=2.9e-36

Best Hits

Swiss-Prot: 88% identical to LEU1_PARD8: 2-isopropylmalate synthase (leuA) from Parabacteroides distasonis (strain ATCC 8503 / DSM 20701 / CIP 104284 / JCM 5825 / NCTC 11152)

KEGG orthology group: K01649, 2-isopropylmalate synthase [EC: 2.3.3.13] (inferred from 88% identity to pdi:BDI_2064)

Predicted SEED Role

"2-isopropylmalate synthase (EC 2.3.3.13)" in subsystem Branched-Chain Amino Acid Biosynthesis or Leucine Biosynthesis (EC 2.3.3.13)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.3.3.13

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (498 amino acids)

>HMPREF1078_RS16665 2-isopropylmalate synthase (Parabacteroides merdae CL09T00C40)
MSDRLFIFDTTLRDGEQVPGCQLNSIEKIQVAKALEELGVDVIEAGFPVSSPGDFNSVVE
ISKAVTWPTICALTRAVEKDIDVAAEALKYAKHGRIHTGIGTSDYHIRYKFNSNQEDILE
RAVAAAKYAKRYVEDVEFYCEDAGRTDNEYLARVVEAVIKAGATVVNIPDTTGYCLPDEY
GAKIKYLMDHVDGIENAIISTHCHNDLGMATANTVQGVLNGARQVEVTMNGIGERAGNTS
LEEIAMIFKSHKERGIITNINTTKIYGVSRMVSSLMNMPIQPNKAIVGRNAFAHSSGIHQ
DGVLKNRESYEIIDPKDVGIDDNAIVLTARSGRAALKHRLQVLGVELSQEKLDKVYEEFL
KLADRKKDINDDDILMLAGKDRTAMHRIKLEYLQVTSGVGLQSVASICLNIAGERFEAAA
SGNGPVDAAIKAVKSVIHRTMTIQEFLIQAINKGSDDMGKVHMQVEYEGNRYYGFAANTD
IIAASVEAFIDAINKFVK