Protein Info for HMPREF1078_RS16660 in Parabacteroides merdae CL09T00C40

Annotation: 3-isopropylmalate dehydratase large subunit

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR00170: 3-isopropylmalate dehydratase, large subunit
  • amino acids 2 to 462 (461 residues), 636.9 bits, E=9.1e-196
• PF00330: Aconitase
  • amino acids 6 to 455 (450 residues), 557 bits, E=2.1e-171

Best Hits

Swiss-Prot: 86% identical to LEUC_BACFR: 3-isopropylmalate dehydratase large subunit (leuC) from Bacteroides fragilis (strain YCH46)

KEGG orthology group: K01703, 3-isopropylmalate/(R)-2-methylmalate dehydratase large subunit [EC: 4.2.1.33 4.2.1.35] (inferred from 86% identity to bfs:BF3266)

MetaCyc: 52% identical to isopropylmalate isomerase large subunit (Leptospira interrogans serovar Lai str. 56601)
(R)-2-methylmalate dehydratase. [EC: 4.2.1.35]; 3-isopropylmalate dehydratase. [EC: 4.2.1.35, 4.2.1.33]; 4.2.1.33 [EC: 4.2.1.35, 4.2.1.33]; RXN-7744 [EC: 4.2.1.35, 4.2.1.33]

Predicted SEED Role

"3-isopropylmalate dehydratase large subunit (EC 4.2.1.33)" in subsystem Branched-Chain Amino Acid Biosynthesis or Leucine Biosynthesis (EC 4.2.1.33)

MetaCyc Pathways

• superpathway of branched chain amino acid biosynthesis (16/17 steps found)
• L-leucine biosynthesis (6/6 steps found)
• 3-methylbutanol biosynthesis (engineered) (5/7 steps found)
• L-isoleucine biosynthesis II (5/8 steps found)
• 2,5-xylenol and 3,5-xylenol degradation (1/13 steps found)

KEGG Metabolic Maps

• Biosynthesis of alkaloids derived from histidine and purine
• C5-Branched dibasic acid metabolism
• Valine, leucine and isoleucine biosynthesis

Isozymes

Compare fitness of predicted isozymes for: 4.2.1.33, 4.2.1.35

Use Curated BLAST to search for 4.2.1.33 or 4.2.1.35

Protein Sequence (464 amino acids)

>HMPREF1078_RS16660 3-isopropylmalate dehydratase large subunit (Parabacteroides merdae CL09T00C40)
MNTLFDKIWDSHVVTMIEEGPTQLYIDRLYCHEVTSPQAFASMRRRGLKPFRPERIYCMP
DHNTPTHDQDKPIEDPVSKNQVDTLAKNAAEFGLTHYGMMDERNGIIHVVGPERGLTLPG
MTIVCGDSHTSTHGAVGAVAFGIGTSEVEMVMASQCILQAKPKTMRICVEGNLGKGVTAK
DVALYIMSKVTTSGATGYFIEYAGAAIRSLTMEGRLTLCNLSIEMGARGGMIAPDETTFA
YLKGRENAPAGEEWDKAVAYWKTLKSGDDAVFDKEILFDAADIEPMVTYGTNPGMGMGVT
EHIPTVEGMNDVEKASFMKSMEYMGFEPGESLLGKKIDYVFLGSCTNGRIEDFRAFASIA
KGRKKADHVVAWLVPGSWLVDAQIREEGLDKIFEEAGFRLRQPGCSACLAMNDDKIPAGK
YAVSTSNRNFEGRQGPGARTILAGPLVAAAAAVTGEITDPRTFF