Protein Info for HMPREF1078_RS16650 in Parabacteroides merdae CL09T00C40

Annotation: alpha-isopropylmalate synthase regulatory domain-containing protein

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 502 PF00682: HMGL-like" amino acids 2 to 268 (267 residues), 173.4 bits, see alignment E=7.3e-55 PF08502: LeuA_dimer" amino acids 364 to 500 (137 residues), 93.7 bits, see alignment E=9e-31

Best Hits

KEGG orthology group: K09011, D-citramalate synthase [EC: 2.3.1.182] (inferred from 88% identity to pdi:BDI_2061)

Predicted SEED Role

"(R)-citramalate synthase (EC 2.3.1.182)" in subsystem Branched-Chain Amino Acid Biosynthesis (EC 2.3.1.182)

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.3.1.182

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (502 amino acids)

>HMPREF1078_RS16650 alpha-isopropylmalate synthase regulatory domain-containing protein (Parabacteroides merdae CL09T00C40)
MIEIMDTTLRDGEQTSGVSFAAHEKLSIAQALLDLGVNRLEIASARVSDGEFEAVKRVAS
WAERTGNIQKLEVLGFVDGDVSLNWIEAAGCRVVNLLCKGSYKHVTEQLRKTPEQHFADI
RSVINQAIERGIAVNIYLEDWSNGIKNSPEYVFQAVDSLRDLPIRRFMLPDTLGILNPGN
TYEYCKEMVTRYPDLKFDFHAHNDYDLAVANVYSAVRAGIKGLHTTLNGLGERAGNAPLS
SVLAVLKDQLGVDTTLREERINYASRLVETFSGVHIPPNKPIIGEHVFTQCAGVHADGDS
KNNLYCNDLLPERFGRVREYALGKTSGKANIRKNLEALGIDIDENSMRKVTERIIELGDK
KEMVTTEDLPYIISDVLHHDTMADQRIRILNYSLSLAQGLKPVATLKIEINGEAYQESAS
GDGQYDAFVRALRKIYTDLGRPFPMLTNYSVSIPPGGRTDAFVQTIISWNYAGVDFKTRG
LDADQTEAAIKATLKMLNKIEQ