Protein Info for HMPREF1078_RS14955 in Parabacteroides merdae CL09T00C40

Annotation: methionine synthase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR02082: methionine synthase
  • amino acids 10 to 1199 (1190 residues), 1562.7 bits, E=0
• PF02574: S-methyl_trans
  • amino acids 15 to 319 (305 residues), 309.8 bits, E=6.9e-96
• PF00809: Pterin_bind
  • amino acids 358 to 596 (239 residues), 200.1 bits, E=1.1e-62
• PF02607: B12-binding_2
  • amino acids 657 to 729 (73 residues), 82.4 bits, 5.5e-27
• PF02310: B12-binding
  • amino acids 746 to 839 (94 residues), 73.7 bits, 3e-24
• PF02965: Met_synt_B12
  • amino acids 935 to 1215 (281 residues), 339.9 bits, E=3e-105

Predicted SEED Role

"5-methyltetrahydrofolate--homocysteine methyltransferase (EC 2.1.1.13)" in subsystem Methionine Biosynthesis (EC 2.1.1.13)

MetaCyc Pathways

• aspartate superpathway (19/25 steps found)
• folate transformations III (E. coli) (7/9 steps found)
• superpathway of S-adenosyl-L-methionine biosynthesis (7/9 steps found)
• superpathway of L-methionine biosynthesis (transsulfuration) (7/9 steps found)
• superpathway of L-methionine biosynthesis (by sulfhydrylation) (9/12 steps found)
• superpathway of L-homoserine and L-methionine biosynthesis (6/8 steps found)
• L-methionine biosynthesis III (3/4 steps found)
• folate transformations II (plants) (8/11 steps found)
• L-methionine biosynthesis I (3/5 steps found)
• superpathway of L-lysine, L-threonine and L-methionine biosynthesis I (12/18 steps found)
• L-methionine salvage from L-homocysteine (1/3 steps found)
• folate transformations I (7/13 steps found)
• superpathway of L-methionine salvage and degradation (7/16 steps found)

KEGG Metabolic Maps

• Methionine metabolism
• One carbon pool by folate

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.1.1.13

Protein Sequence (1231 amino acids)

>HMPREF1078_RS14955 methionine synthase (Parabacteroides merdae CL09T00C40)
MDKEIFLNLLKERILILDGGMGTMVQGFKLTEKDYRGKQFADWMSDLKGNNDLLCITRPD
VIKSIHRQYLDAGADIFATNTFNANAISMEDYGMQGQVRNINLAAGKLAREVADGFMKEH
PDRTIFVAGSVGPTNKTASMSPDVSDPAYRAVTYLDLYSAYKEQVDALVDGGVDIVLFET
TFDTLNVKAGLEAAEAVLKEKGKDLPIMLSLTLSAQGGRTFSGQTLLAFLASVQHTNIVS
VGLNCSFGAADMKPFLAELAKHAPYYISAYPNAGLPNSFGSYDETPEKMAVHVKSFIDEG
LVNILGGCCGTTPAHIAKYPELVKGAKPHVPAPRPDCLWLSGLELLEVKPENNFVNVGER
CNVAGSRKFLRLIKEENYEEALTIARKQVEDGAQVIDINMDDGMLDAVKEMTTFLNLIAS
EPDISRVPVMIDSSKWEVIEKGLMCVQGKSIVNSISLKEGEEVFLQHAARIKQLGAATVV
MAFDEKGQADTFERKIEVCGRAYRLLREKVDFDPNGIIFDPNVLAIATGMEEHNGYGLDF
IRAVEWIKKNLPGAKVSGGVSNLSFSFRGNNYVREAMHSVFLYHAINKGMDMGIVNPSSS
VLYEDIEPAFRMLLEDVILACRPEAAEELIAYAQNLHVKAQEGEGEKQEVWREYPLKERL
ENALIKGVGDHLEEDLKEALKEYPRAVDIIDGPLMGGMNKVGELFGAGKMFLPQVVKTAR
TMKKAVAILQPAIEAEKVSSDSAKAGKVLFATVKGDVHDIGKNIVSIVLACNNYEVIDLG
VMVPADVIVKKAIEEKPDLVCLSGLITPSLEEMVHVTDEMQKAGLSIPIMVGGATTSKLH
TAIKIAPHYDYPVIHVLDASQNPLIAAKLLNPDTRDAYIAQLNSEYEALRASVNKKKEIL
VPLAEARTNRPVIDWASYNPVVPARNGVQVIPSIPLEEIIPYIHWTFFFSAWKLNGRFSE
IAQIHGCDACRAAWLAEFSEADRTKASEAMQLYKDAVKLLDRLVEMKAEYCKAVYGFFPA
NGDGDNIRVGEVLLPVLRQQAKKEEGIYKSLADYVMPVSEGRTDYVGAFVVTAGTGAESL
KERFEQEGDTYNSMLLQTLTDRLAEATAEYLHEKVRKEYWGYAPDESLSISDLYKVKYQG
IRPAIGYPSLPDQLLNYTLDKLLNMSQIGVRLTENGAMYPTATVSGIYIAHPDSQYFMIG
NIDEEQMKDYACRRNLSEAEVKKLLNKNISN