Protein Info for HMPREF1078_RS14205 in Parabacteroides merdae CL09T00C40

Annotation: bifunctional demethylmenaquinone methyltransferase/2-methoxy-6-polyprenyl-1,4-benzoquinol methylase UbiE

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 255 PF01209: Ubie_methyltran" amino acids 17 to 246 (230 residues), 214.2 bits, see alignment E=5.1e-67 TIGR01934: ubiquinone/menaquinone biosynthesis methyltransferase" amino acids 22 to 246 (225 residues), 257.2 bits, see alignment E=5.3e-81 PF13489: Methyltransf_23" amino acids 52 to 176 (125 residues), 41.5 bits, see alignment E=3.6e-14 PF13847: Methyltransf_31" amino acids 63 to 172 (110 residues), 62.2 bits, see alignment E=1.3e-20 PF13649: Methyltransf_25" amino acids 64 to 160 (97 residues), 81.9 bits, see alignment E=1.3e-26 PF08242: Methyltransf_12" amino acids 65 to 162 (98 residues), 52.7 bits, see alignment E=1.8e-17 PF08241: Methyltransf_11" amino acids 65 to 164 (100 residues), 73.3 bits, see alignment E=6.4e-24

Best Hits

Swiss-Prot: 60% identical to MENG_BACFR: Demethylmenaquinone methyltransferase (menG) from Bacteroides fragilis (strain YCH46)

KEGG orthology group: K03183, ubiquinone/menaquinone biosynthesis methyltransferase [EC: 2.1.1.- 2.1.1.163] (inferred from 82% identity to pdi:BDI_1988)

MetaCyc: 33% identical to 2-methoxy-6-polyprenyl-1,4-benzoquinol methylase (Cereibacter sphaeroides)
RXN-9235 [EC: 2.1.1.201]

Predicted SEED Role

No annotation

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 2.1.1.-

Use Curated BLAST to search for 2.1.1.- or 2.1.1.163 or 2.1.1.201

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (255 amino acids)

>HMPREF1078_RS14205 bifunctional demethylmenaquinone methyltransferase/2-methoxy-6-polyprenyl-1,4-benzoquinol methylase UbiE (Parabacteroides merdae CL09T00C40)
MAKYGSEQILPYNNEEHKTTQVRRMFDSIAGTYDQLNHTLSLGIDKIWRRKGIAFLRPFS
PASILDIATGTGDLAISMHRRLKADRIIGADISEGMMEVGRQKVAEAGLSDHITFEYQDC
TALTYPDNSFDAVTAAFGVRNFENIEQGITEMYRVLKPGGHLMILELSSPVHFPMKQLYN
IYSKVVIPTVGRLLSKEHTAYSYLPASIKVVPQGKVMTDLLSRVGFSEARSHTFTFGICS
LYTGSKGGSQTTPTV