Protein Info for HMPREF1078_RS14095 in Parabacteroides merdae CL09T00C40

Annotation: molecular chaperone DnaK

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 638 TIGR02350: chaperone protein DnaK" amino acids 3 to 597 (595 residues), 981.1 bits, see alignment E=8.7e-300 PF00012: HSP70" amino acids 4 to 597 (594 residues), 882.2 bits, see alignment E=1.5e-269 PF06723: MreB_Mbl" amino acids 116 to 370 (255 residues), 51.9 bits, see alignment E=5.4e-18

Best Hits

Swiss-Prot: 96% identical to DNAK_PARD8: Chaperone protein DnaK (dnaK) from Parabacteroides distasonis (strain ATCC 8503 / DSM 20701 / CIP 104284 / JCM 5825 / NCTC 11152)

KEGG orthology group: K04043, molecular chaperone DnaK (inferred from 96% identity to pdi:BDI_1997)

MetaCyc: 61% identical to chaperone protein DnaK (Escherichia coli K-12 substr. MG1655)
Non-chaperonin molecular chaperone ATPase. [EC: 3.6.4.10, 5.6.1.7]

Predicted SEED Role

"Chaperone protein DnaK" in subsystem Heat shock dnaK gene cluster extended or Protein chaperones

Isozymes

No predicted isozymes

Use Curated BLAST to search for 3.6.4.10 or 5.6.1.7

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (638 amino acids)

>HMPREF1078_RS14095 molecular chaperone DnaK (Parabacteroides merdae CL09T00C40)
MGKIIGIDLGTTNSCVAVLEGNEPVVIANSEGKRTTPSIVAFVEGGERKVGDPAKRQAIT
NPEKTIFSIKRFMGETYDQVQKEIGRVPYKVVRGDNNTPRVDIEGRLYTPQEISAMVLQK
MKKTAEDYLGQEVTEAVITVPAYFSDAQRQATKEAGEIAGLNVRRIVNEPTAASLAYGLD
KTNKDMKIAVFDLGGGTFDISILELGDGVFEVKSTNGDTHLGGDDFDHVIIDWLAEEFLR
EEGVDLRKDPMALQRLKEAAEKAKIELSSTTSTEINLPYIMPVNGVPKHLVKTLTRAKFE
QLADSLIQACIEPCRQSLKDAGLSTSDIDEVILVGGSTRIPAVQAIVEKFFGKAPSKGVN
PDEVVAVGAAIQGGVLTGEVKDVLLLDVTPLSLGIETMGGVMTKLIEANTTIPTKKSETF
TTAVDNQPSVEIHVLQGERSLAKDNKSIGRFHLDGIPAAQRGVPQIEVTFDIDANGILNV
SAKDKGTGKVQSIRIEASSGLSDDEVKRMKEEAQANAEADKKEKERIDKLNQADSMIFQT
EKQLKDLGDKLPADKKAPIETALNKLKEAHKAQDIAGIDAAMAELNSVFQAASQEMYNAQ
NAQGGAQPGPDFGQQTGGNAGNNKQDGGVTDVDFEEVK