Protein Info for HMPREF1078_RS13575 in Parabacteroides merdae CL09T00C40

Annotation: imidazole glycerol phosphate synthase subunit HisH

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 196 PF07685: GATase_3" amino acids 2 to 102 (101 residues), 27.6 bits, see alignment E=3.3e-10 TIGR01855: imidazole glycerol phosphate synthase, glutamine amidotransferase subunit" amino acids 3 to 195 (193 residues), 208.6 bits, see alignment E=4.2e-66 PF00117: GATase" amino acids 10 to 194 (185 residues), 69.4 bits, see alignment E=5e-23 PF01174: SNO" amino acids 15 to 106 (92 residues), 30.1 bits, see alignment E=6.6e-11

Best Hits

Swiss-Prot: 73% identical to HIS5_BACFR: Imidazole glycerol phosphate synthase subunit HisH (hisH) from Bacteroides fragilis (strain YCH46)

KEGG orthology group: K02501, glutamine amidotransferase [EC: 2.4.2.-] (inferred from 85% identity to pdi:BDI_2016)

Predicted SEED Role

"Imidazole glycerol phosphate synthase amidotransferase subunit (EC 2.4.2.-)" in subsystem Histidine Biosynthesis (EC 2.4.2.-)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 2.4.2.-

Use Curated BLAST to search for 2.4.2.-

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (196 amino acids)

>HMPREF1078_RS13575 imidazole glycerol phosphate synthase subunit HisH (Parabacteroides merdae CL09T00C40)
MDVAIIKYNAGNIYSVSYALKRLGVEATITADPELLCKADKVIFPGVGEAHTTMEHLKEH
NLDTIIKGLKQPVLGICLGMQLMCRHSEEGDANCLGIFDTEVKRFIPQQHMDKVPHMGWN
TITNVKGGLFNKQLENKFVYFVHSYYVPVNEYTAATTEYILPFSASLHKDNFYATQFHPE
KSGSVGEVILSNFLKL