Protein Info for HMPREF1078_RS13560 in Parabacteroides merdae CL09T00C40

Annotation: bifunctional phosphoribosyl-AMP cyclohydrolase/phosphoribosyl-ATP diphosphatase HisIE

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF01502: PRA-CH
  • amino acids 26 to 98 (73 residues), 109.9 bits, E=4.5e-36
• TIGR03188: phosphoribosyl-ATP diphosphatase
  • amino acids 109 to 192 (84 residues), 104.6 bits, E=1.2e-34
• PF01503: PRA-PH
  • amino acids 110 to 196 (87 residues), 71.8 bits, E=4.9e-24

Best Hits

Swiss-Prot: 76% identical to HIS2_BACTN: Histidine biosynthesis bifunctional protein HisIE (hisI) from Bacteroides thetaiotaomicron (strain ATCC 29148 / DSM 2079 / NCTC 10582 / E50 / VPI-5482)

KEGG orthology group: K11755, phosphoribosyl-ATP pyrophosphohydrolase / phosphoribosyl-AMP cyclohydrolase [EC: 3.5.4.19 3.6.1.31] (inferred from 89% identity to pdi:BDI_2019)

MetaCyc: 52% identical to putative bifunctional phosphoribosyl-AMP cyclohydrolase/phosphoribosyl-ATP pyrophosphatase (Escherichia coli K-12 substr. MG1655)
Phosphoribosyl-ATP diphosphatase. [EC: 3.6.1.31]; Phosphoribosyl-AMP cyclohydrolase. [EC: 3.6.1.31, 3.5.4.19]

Predicted SEED Role

"Phosphoribosyl-AMP cyclohydrolase (EC 3.5.4.19) / Phosphoribosyl-ATP pyrophosphatase (EC 3.6.1.31)" in subsystem Histidine Biosynthesis (EC 3.5.4.19, EC 3.6.1.31)

MetaCyc Pathways

• superpathway of histidine, purine, and pyrimidine biosynthesis (38/46 steps found)
• L-histidine biosynthesis (10/10 steps found)

KEGG Metabolic Maps

• Biosynthesis of alkaloids derived from histidine and purine
• Biosynthesis of plant hormones
• Histidine metabolism

Isozymes

No predicted isozymes

Use Curated BLAST to search for 3.5.4.19 or 3.6.1.31

Protein Sequence (198 amino acids)

>HMPREF1078_RS13560 bifunctional phosphoribosyl-AMP cyclohydrolase/phosphoribosyl-ATP diphosphatase HisIE (Parabacteroides merdae CL09T00C40)
MKLDFEKMGGLIPAIVQDYNTNKVLMLGFMNEEAYEETKATGKVTFFSRTKNRIWMKGET
SGNTLQVVTIAADCDNDTLLIKAIPAGPVCHTGADTCFGEKNVEDIMFLKYLQNFIEQRR
QEMPEGSYTTTLFQKGINRMAQKVGEEAVETVIEATNGTDDRLVYEAADMIYHLIVLLTS
KGLRIEDLARELKSRHKG