Protein Info for HMPREF1078_RS13320 in Parabacteroides merdae CL09T00C40

Annotation: NADP-specific glutamate dehydrogenase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF02812: ELFV_dehydrog_N
  • amino acids 53 to 181 (129 residues), 172 bits, E=4.8e-55
• PF00208: ELFV_dehydrog
  • amino acids 198 to 442 (245 residues), 283.4 bits, E=1.8e-88

Best Hits

Swiss-Prot: 85% identical to DHE2_BACFR: NAD-specific glutamate dehydrogenase (gdhB) from Bacteroides fragilis (strain YCH46)

KEGG orthology group: K00262, glutamate dehydrogenase (NADP+) [EC: 1.4.1.4] (inferred from 97% identity to pdi:BDI_3804)

MetaCyc: 58% identical to glutamate dehydrogenase (Escherichia coli K-12 substr. MG1655)
Glutamate dehydrogenase (NADP(+)). [EC: 1.4.1.4]

Predicted SEED Role

"NAD-specific glutamate dehydrogenase (EC 1.4.1.2)" in subsystem Glutamate dehydrogenases or Glutamine, Glutamate, Aspartate and Asparagine Biosynthesis (EC 1.4.1.2)

MetaCyc Pathways

• L-glutamate biosynthesis III (1/1 steps found)
• L-glutamate degradation I (1/1 steps found)
• L-glutamate and L-glutamine biosynthesis (5/7 steps found)
• L-alanine degradation II (to D-lactate) (2/3 steps found)
• ethene biosynthesis IV (engineered) (1/3 steps found)
• L-glutamate degradation XI (reductive Stickland reaction) (2/7 steps found)
• methylaspartate cycle (10/19 steps found)
• 4-aminobutanoate degradation V (1/7 steps found)
• L-glutamate degradation V (via hydroxyglutarate) (2/10 steps found)

KEGG Metabolic Maps

• Arginine and proline metabolism
• Biosynthesis of alkaloids derived from ornithine, lysine and nicotinic acid
• Glutamate metabolism
• Nitrogen metabolism

Isozymes

Compare fitness of predicted isozymes for: 1.4.1.2, 1.4.1.4

Use Curated BLAST to search for 1.4.1.2 or 1.4.1.4

Protein Sequence (444 amino acids)

>HMPREF1078_RS13320 NADP-specific glutamate dehydrogenase (Parabacteroides merdae CL09T00C40)
MKTEVILSALEAKHPGEKEYLQAVKEVLLSIEEVYNQHPEFEKAKIIERLVEPERIFTFR
VPWVDDKGEIQVNLGYRVQFNNAIGPYKGGIRFHPSVNLSILKFLGFEQTFKNALTTLPM
GGGKGGSDFAPRGKSDAEIMRFCQAFILELWRNLGPDRDVPAGDIGVGGREVGYMYGMYK
KLARENTGTFTGKGMEFGGSILRPEATGFGALYFVHQMLETHGIDIKGKTVAISGFGNVA
WGAATKATELGAKVVTISGPDGYIYDPDGISGKKIDYMLELRNSGNDIVAPYAEEFPGST
FYPGKKPWEQKVDIALPCATQNELDADDARKLIENKTSCVAEVSNMGCTAEAVDLFIEHK
QLFAPGKAVNAGGVATSGLEMTQNAMHISWTAAEVDDKLHQIMSAIHQQCVEHGTEDQYI
NYVKGANIAGFMKVAKAMMAQGIV