Protein Info for HMPREF1078_RS11400 in Parabacteroides merdae CL09T00C40

Annotation: NADPH-dependent glutamate synthase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF14691: Fer4_20
  • amino acids 48 to 155 (108 residues), 139 bits, E=1.8e-44
• TIGR01316: glutamate synthase (NADPH), homotetrameric
  • amino acids 48 to 486 (439 residues), 565.3 bits, E=4.6e-174
• PF00070: Pyr_redox
  • amino acids 170 to 242 (73 residues), 26.8 bits, E=1.8e-09
  • amino acids 309 to 385 (77 residues), 28.3 bits, E=6.2e-10
• PF07992: Pyr_redox_2
  • amino acids 170 to 476 (307 residues), 118 bits, E=1.8e-37
• PF13450: NAD_binding_8
  • amino acids 173 to 207 (35 residues), 33.6 bits, 1.2e-11

Best Hits

KEGG orthology group: K00266, glutamate synthase (NADPH/NADH) small chain [EC: 1.4.1.13 1.4.1.14] (inferred from 93% identity to pdi:BDI_3682)

MetaCyc: 60% identical to NAD(P)+ transhydrogenase (ferredoxin) beta subunit (Clostridium kluyveri)
RXN-16837 [EC: 1.6.1.4]

Predicted SEED Role

"Glutamate synthase [NADPH] small chain (EC 1.4.1.13)" in subsystem Ammonia assimilation or Glutamine, Glutamate, Aspartate and Asparagine Biosynthesis (EC 1.4.1.13)

MetaCyc Pathways

• ammonia assimilation cycle III (3/3 steps found)
• L-glutamate biosynthesis I (2/2 steps found)
• ammonia assimilation cycle I (2/2 steps found)
• L-glutamate biosynthesis IV (1/1 steps found)
• L-glutamine degradation I (1/1 steps found)
• L-glutamine degradation II (1/1 steps found)
• L-citrulline biosynthesis (6/8 steps found)
• L-glutamate and L-glutamine biosynthesis (5/7 steps found)
• superpathway of ammonia assimilation (plants) (2/3 steps found)
• superpathway of L-citrulline metabolism (8/12 steps found)
• L-asparagine biosynthesis III (tRNA-dependent) (1/4 steps found)
• glutaminyl-tRNA^gln biosynthesis via transamidation (1/4 steps found)

KEGG Metabolic Maps

• Biosynthesis of alkaloids derived from ornithine, lysine and nicotinic acid
• Glutamate metabolism
• Nitrogen metabolism

Isozymes

Compare fitness of predicted isozymes for: 1.4.1.13, 1.4.1.14

Use Curated BLAST to search for 1.4.1.13 or 1.4.1.14 or 1.6.1.4

Protein Sequence (489 amino acids)

>HMPREF1078_RS11400 NADPH-dependent glutamate synthase (Parabacteroides merdae CL09T00C40)
MTTEELIAARRAEPWREALRKSKKNKERTDIPRVEMNELDAEYRSHTRLEEVNLGLTKEQ
AMQEAQRCLDCPNPTCMQGCPVSINIPTFVKNIERGEFLEAARVLKETSALPAVCGRVCP
QEKQCESKCIHLKMGKPAVAIGYLERFAADYERESGNISIPEVAEKNGIKIAVVGSGPAG
LSFAGDMAKRGYDVTVFEALHEIGGVLKYGIPEFRLPNKIVDVEIEGLRKMGVKFMTNCI
VGKTISYDDLHADGFKGIFAASGAGLPNFMNIPGENLVGVMSSNEYLTRVNLMDAANPDS
DTPVLQGKKVAVIGGGNTAMDSVRTARRLGAERAMIVYRRSEEEMPARLEEVKHAKEEGV
EFMTLHNPVEYLGDERGRVKQMRLQKMELGEPDASGRRRPVPVEGAIETIDVDEVIVSVG
VSPNPLIPRAFQGLEVSKKGTIVVNEENMRSALPDVYAGGDIVRGGATVILAMGDGRKAA
AAMDEALRG