Protein Info for HMPREF1078_RS10685 in Parabacteroides merdae CL09T00C40

Annotation: tRNA (N(6)-L-threonylcarbamoyladenosine(37)-C(2))- methylthiotransferase MtaB

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 440 PF00919: UPF0004" amino acids 14 to 106 (93 residues), 87.2 bits, see alignment E=6.3e-29 TIGR00089: radical SAM methylthiotransferase, MiaB/RimO family" amino acids 16 to 427 (412 residues), 366.1 bits, see alignment E=2.4e-113 TIGR01579: MiaB-like tRNA modifying enzyme" amino acids 16 to 422 (407 residues), 399.9 bits, see alignment E=1.7e-123 PF04055: Radical_SAM" amino acids 155 to 323 (169 residues), 70.1 bits, see alignment E=2.7e-23

Best Hits

KEGG orthology group: None (inferred from 89% identity to pdi:BDI_0048)

Predicted SEED Role

"tRNA-t(6)A37 methylthiotransferase"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (440 amino acids)

>HMPREF1078_RS10685 tRNA (N(6)-L-threonylcarbamoyladenosine(37)-C(2))- methylthiotransferase MtaB (Parabacteroides merdae CL09T00C40)
MIDKTVFENKIAAYYTLGCKLNFAETSTIGKVLAEQGVRKARPGEKADICVVNTCSVTEL
ADKKCRQAIRRIGKQHPGAFIVVTGCYAQLKPEEVSHIEGVDLVLGAEQKLDLLMYLDDL
KKREEGGAIIASRTKDIRTFSPSCSADDRTRHFLKVQDGCDYFCSYCTIPFARGRSRNGT
IASMVKQAEEVASNGGKEIVLTGVNIGDFGKSTGETFIDLIRALDEVEGIVRYRISSIEP
NLITDEAIDFVACSRRFAPHFHIPLQSGSDEVLKLMRRRYDTILFRHKIEKIKEVMPHAF
IGVDVIVGTRGETDTCFEEARTFIESLDISQLHVFSYSERPGTQALKIDHVVDPKTKHAR
SQQLLDISDRKLHAFYEAHIGQKANVLFEQTRKGGMMHGFTENYIKVEIPYDHSLVNETR
QVILEGWNEDKTALVVKIIE