Protein Info for HMPREF1078_RS10535 in Parabacteroides merdae CL09T00C40

Annotation: large-conductance mechanosensitive channel protein MscL

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 140 transmembrane" amino acids 21 to 50 (30 residues), see Phobius details amino acids 83 to 102 (20 residues), see Phobius details PF01741: MscL" amino acids 4 to 137 (134 residues), 157.5 bits, see alignment E=9.8e-51 TIGR00220: large conductance mechanosensitive channel protein" amino acids 4 to 139 (136 residues), 156.5 bits, see alignment E=2e-50

Best Hits

Swiss-Prot: 84% identical to MSCL_PARD8: Large-conductance mechanosensitive channel (mscL) from Parabacteroides distasonis (strain ATCC 8503 / DSM 20701 / CIP 104284 / JCM 5825 / NCTC 11152)

KEGG orthology group: K03282, large conductance mechanosensitive channel (inferred from 84% identity to pdi:BDI_0117)

MetaCyc: 59% identical to large conductance mechanosensitive channel (Escherichia coli K-12 substr. MG1655)
TRANS-RXN-86

Predicted SEED Role

"Large-conductance mechanosensitive channel" in subsystem Potassium homeostasis

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (140 amino acids)

>HMPREF1078_RS10535 large-conductance mechanosensitive channel protein MscL (Parabacteroides merdae CL09T00C40)
MKKILQEFKQFAMRGNVIDMAVGIIIGGAFGKIVSSVVGDIIMPAIGLLIGGVNFTDLKI
VLKQAVMDGDKVVSPAVSINYGNFLQVTFDFIIIAFSIFMLVKGINALNKKKEEAPAAPA
PPPADIQLLTEIRDLLKDKK