Protein Info for HMPREF1078_RS10390 in Parabacteroides merdae CL09T00C40

Annotation: cofactor-independent phosphoglycerate mutase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR02535: proposed homoserine kinase
  • amino acids 1 to 400 (400 residues), 564.7 bits, E=9.4e-174
• PF01676: Metalloenzyme
  • amino acids 1 to 378 (378 residues), 151.1 bits, E=4.1e-48
• TIGR00306: phosphoglycerate mutase (2,3-diphosphoglycerate-independent), archaeal form
  • amino acids 4 to 397 (394 residues), 396.8 bits, E=1.2e-122
• PF10143: PhosphMutase
  • amino acids 37 to 205 (169 residues), 207 bits, E=2e-65

Best Hits

Swiss-Prot: 48% identical to APGM2_METTH: 2,3-bisphosphoglycerate-independent phosphoglycerate mutase 2 (apgM2) from Methanothermobacter thermautotrophicus (strain ATCC 29096 / DSM 1053 / JCM 10044 / NBRC 100330 / Delta H)

KEGG orthology group: K01834, phosphoglycerate mutase [EC: 5.4.2.1] (inferred from 88% identity to pdi:BDI_0260)

MetaCyc: 48% identical to 2,3-bisphosphoglycerate-independent phosphoglycerate mutase 2 (Methanothermobacter thermautotrophicus Delta H)
3PGAREARR-RXN [EC: 5.4.2.12]

Predicted SEED Role

"Predicted functional analog of homoserine kinase (EC 2.7.1.-)" in subsystem Threonine and Homoserine Biosynthesis (EC 2.7.1.-)

MetaCyc Pathways

• superpathway of glucose and xylose degradation (16/17 steps found)
• glycolysis III (from glucose) (11/11 steps found)
• homolactic fermentation (11/12 steps found)
• glycolysis II (from fructose 6-phosphate) (10/11 steps found)
• glycolysis IV (9/10 steps found)
• superpathway of glycolysis and the Entner-Doudoroff pathway (14/17 steps found)
• gluconeogenesis I (11/13 steps found)
• glycolysis I (from glucose 6-phosphate) (11/13 steps found)
• Bifidobacterium shunt (12/15 steps found)
• Rubisco shunt (8/10 steps found)
• Entner-Doudoroff pathway III (semi-phosphorylative) (7/9 steps found)
• superpathway of N-acetylneuraminate degradation (16/22 steps found)
• superpathway of cytosolic glycolysis (plants), pyruvate dehydrogenase and TCA cycle (16/22 steps found)
• glycolysis VI (from fructose) (8/11 steps found)
• hexitol fermentation to lactate, formate, ethanol and acetate (13/19 steps found)
• superpathway of anaerobic sucrose degradation (13/19 steps found)
• superpathway of hexitol degradation (bacteria) (12/18 steps found)
• glycolysis V (Pyrococcus) (6/10 steps found)
• superpathway of glycolysis, pyruvate dehydrogenase, TCA, and glyoxylate bypass (17/26 steps found)
• ethene biosynthesis V (engineered) (16/25 steps found)
• photorespiration II (5/10 steps found)
• glycerol degradation to butanol (8/16 steps found)
• gluconeogenesis II (Methanobacterium thermoautotrophicum) (8/18 steps found)
• photosynthetic 3-hydroxybutanoate biosynthesis (engineered) (13/26 steps found)
• 1-butanol autotrophic biosynthesis (engineered) (13/27 steps found)
• Methanobacterium thermoautotrophicum biosynthetic metabolism (19/56 steps found)

KEGG Metabolic Maps

• Biosynthesis of alkaloids derived from histidine and purine
• Biosynthesis of alkaloids derived from ornithine, lysine and nicotinic acid
• Biosynthesis of alkaloids derived from shikimate pathway
• Biosynthesis of alkaloids derived from terpenoid and polyketide
• Biosynthesis of phenylpropanoids
• Biosynthesis of plant hormones
• Biosynthesis of terpenoids and steroids
• Glycolysis / Gluconeogenesis
• Lipopolysaccharide biosynthesis
• Nicotinate and nicotinamide metabolism
• Pentose phosphate pathway

Isozymes

Compare fitness of predicted isozymes for: 2.7.1.-, 5.4.2.1, 5.4.2.12

Use Curated BLAST to search for 2.7.1.- or 5.4.2.1 or 5.4.2.12

Protein Sequence (400 amino acids)

>HMPREF1078_RS10390 cofactor-independent phosphoglycerate mutase (Parabacteroides merdae CL09T00C40)
MKSIIILGDGMADEPIEALGGKTPMQYADTPYMDKLAEMGVTGRMKTVADGFHPGSEVAN
MAVLGYDLPTVYEGRGVLEAASIGYDLKPSEMAMRCNLICVEGDILKNHSSGHITTEEAD
ELIRFLNEKLGSDRIHFYTGVSYRHLLVVKGGDKRLDCTPPHDVPLHPFRPLMIKPEAPE
AQETADLLNNLILQSQEILKDHPVNLQRIAAGKDPANSIWPWSPGYRPAMQTMQEMYGFK
QGSVISAVDLIRGIGVYAGLEVIDVEGATGLYDTNYEGKAHAALEALKTNDFVYLHVEAS
DEAGHEGDVDLKIRTIENLDKRAIGILFEELQKWDEPVAIAVLPDHPTPCAIRTHTNTPV
PFLIYKPGQEPDSVTRFDEFSVLEGKHGILEKDEFIKELL