Protein Info for HMPREF1078_RS08615 in Parabacteroides merdae CL09T00C40

Annotation: glutamine--tRNA ligase/YqeY domain fusion protein

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 580 TIGR00440: glutamine--tRNA ligase" amino acids 35 to 576 (542 residues), 687.7 bits, see alignment E=6.2e-211 PF00749: tRNA-synt_1c" amino acids 35 to 348 (314 residues), 339.4 bits, see alignment E=2.7e-105 PF03950: tRNA-synt_1c_C" amino acids 351 to 451 (101 residues), 100.8 bits, see alignment E=6.1e-33 PF20974: tRNA-synt_1c_C2" amino acids 468 to 554 (87 residues), 75.6 bits, see alignment E=6.3e-25

Best Hits

Swiss-Prot: 58% identical to SYQ_RHOBA: Glutamine--tRNA ligase (glnS) from Rhodopirellula baltica (strain DSM 10527 / NCIMB 13988 / SH1)

KEGG orthology group: K01886, glutaminyl-tRNA synthetase [EC: 6.1.1.18] (inferred from 87% identity to pdi:BDI_1388)

Predicted SEED Role

"Glutaminyl-tRNA synthetase (EC 6.1.1.18)" in subsystem tRNA aminoacylation, Glu and Gln (EC 6.1.1.18)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 6.1.1.18

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (580 amino acids)

>HMPREF1078_RS08615 glutamine--tRNA ligase/YqeY domain fusion protein (Parabacteroides merdae CL09T00C40)
MSDIKTNEEEGKKSLNFIEAMVEKDLAEGKNKGRVQTRFPPEPNGYLHIGHAKAICLDFG
IAQKYGGVCNLRFDDTNPVKEDLEYVDAIKEDIEWLGYHWNNIYYASDYFQQLWDFAIQL
IKEGKAYIDEQTSEQIASQKGTPTQPGTNSPYRDRPIEENLELFHKMNSGELEEGSMVLR
AKIDMANPNMHFRDPIIYRIVKTPHHRTGDKWKAYPMYDFAHGQSDFFEGVTHSLCTLEF
VVHRPLYDLFIDWLKEGKDLNDNRPRQTEFNKLNLSYTLMSKRNLLILVKEGLVHDWDDP
RMPTICGFRRRGYSPEAIHKFIDKIGYTTYDALNEFALLESAVREDLNTRATRVSAVLNP
VKLIITNYPEGQVEEMEAINNPEDLSAGSHTIEFSRELWIEREDFMEDAPKKFFRMTPGQ
EVRLKNAYIVKCTGCKKDENGEITEIYCEYDPNTKSGMPDSNRKVKGTLHWLSCAHCLPA
EVRLYDRLWKVENPRDEMAAIREAKNCSPLEAMKEIINPDSLKVLTNCYVEKFLADSKPL
DYLQFQRIGYFNVDKDSTVDKLVFNRTVSLKDTWSKVKDK