Protein Info for HMPREF1078_RS07485 in Parabacteroides merdae CL09T00C40

Annotation: formate--tetrahydrofolate ligase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF01268: FTHFS
  • amino acids 2 to 555 (554 residues), 832.6 bits, E=6.2e-255

Best Hits

Swiss-Prot: 89% identical to FTHS_PARD8: Formate--tetrahydrofolate ligase (fhs) from Parabacteroides distasonis (strain ATCC 8503 / DSM 20701 / CIP 104284 / JCM 5825 / NCTC 11152)

KEGG orthology group: K01938, formate--tetrahydrofolate ligase [EC: 6.3.4.3] (inferred from 89% identity to pdi:BDI_1014)

Predicted SEED Role

"Formate--tetrahydrofolate ligase (EC 6.3.4.3)" in subsystem Folate Biosynthesis or One-carbon metabolism by tetrahydropterines or Serine-glyoxylate cycle (EC 6.3.4.3)

MetaCyc Pathways

• L-histidine degradation III (6/6 steps found)
• folate polyglutamylation (5/5 steps found)
• superpathway of tetrahydrofolate biosynthesis and salvage (10/12 steps found)
• formate assimilation into 5,10-methylenetetrahydrofolate (3/3 steps found)
• tetrahydrofolate salvage from 5,10-methenyltetrahydrofolate (2/2 steps found)
• folate transformations III (E. coli) (7/9 steps found)
• formaldehyde oxidation VII (THF pathway) (3/4 steps found)
• folate transformations II (plants) (8/11 steps found)
• reductive glycine pathway of autotrophic CO₂ fixation (6/9 steps found)
• folate transformations I (7/13 steps found)
• reductive acetyl coenzyme A pathway I (homoacetogenic bacteria) (3/10 steps found)
• purine nucleobases degradation II (anaerobic) (12/24 steps found)
• purine nucleobases degradation I (anaerobic) (5/15 steps found)
• Methanobacterium thermoautotrophicum biosynthetic metabolism (19/56 steps found)

KEGG Metabolic Maps

• Glyoxylate and dicarboxylate metabolism
• One carbon pool by folate

Isozymes

No predicted isozymes

Use Curated BLAST to search for 6.3.4.3

Protein Sequence (555 amino acids)

>HMPREF1078_RS07485 formate--tetrahydrofolate ligase (Parabacteroides merdae CL09T00C40)
MKSDIEIAREVSLRKIKEIATGLGIPREEVQNYGRYIAKIPIHLIDEEKIRQHNLILVTA
ITPTKAGIGKTTVSIGLALGLNKIGKKAVVALREPSLGPCFGMKGGAAGGGYSQVLPMEN
INLHFTGDFHAVTSAHNMITALLDNYIYQTRNTCEGLKEIKWKRVLDVNDRSLRNIVSGL
GGSANGVPTETGFDITPASEIMAILCLATDIEDLKRRIGNILLGYTNDDKPFTVNDLGVA
GAITVLLKDALLPNLVQTTENTAAFVHGGPFANIAHGCNSVLATKMALTYGDYVITEAGF
GADLGAEKFFDIKCRKAGLTPKLTVIVATAQSLKLHGGVPENKIKEQNIEGMKNGFENLD
KHVENMKRFGQEVIVTFNRYASDTDEEIALVAEHCREIGVGFCMNNVFAAGGEGGAELAK
LVVDTIEKKPSTPLKYIYEDDESIRSKIKKVSEQIYGAASVVYTTLADKKIKQIESLGIS
HYPICIAKTQYSFSSDPKAYGVAKNFELKVRDIIINNGAEMIVVIMGEIMRMPGLPKDPQ
AKRIDIVDGVIEGLS