Protein Info for HMPREF1078_RS07450 in Parabacteroides merdae CL09T00C40

Annotation: cobalt-precorrin-5B (C(1))-methyltransferase CbiD

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 614 PF02571: CbiJ" amino acids 2 to 229 (228 residues), 178.6 bits, see alignment E=1.5e-56 PF01888: CbiD" amino acids 252 to 524 (273 residues), 287.8 bits, see alignment E=6.7e-90 TIGR00312: cobalamin biosynthesis protein CbiD" amino acids 259 to 610 (352 residues), 204.9 bits, see alignment E=7.7e-65

Best Hits

Predicted SEED Role

"Cobalt-precorrin-6x reductase (EC 1.3.1.54) / Cobalt-precorrin-6 synthase, anaerobic" in subsystem Cobalamin synthesis or Coenzyme B12 biosynthesis (EC 1.3.1.54)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 1.3.1.54

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (614 amino acids)

>HMPREF1078_RS07450 cobalt-precorrin-5B (C(1))-methyltransferase CbiD (Parabacteroides merdae CL09T00C40)
MILILGGTTEGRKAVQVVESAGKPYYYSTVGNEQAIEAVHAIRLTGGMDEEKMAQFCREK
KISLLVDAAHPFAIRLHRTLAKVSSRLRIPVVRLERQYPAHDKRLIWCTDYAAAIDRLRA
DGICNLLALTGVKTIAKLRPYWEQTPCWFRILNRKESLSLAESDGFPKERIIFFHEGEDE
KKLLDQLHPDAVLTKESGESGYFKEKVEAAQACGIPVYVIQRPPLPDSFTFVQGMEGLRK
AIERLAPGFFPLRSGFTTGTCATAAAKAALVALLNRTEQTVSCITLPSGEKIMLPVAATE
WADNAALCTVIKDSGDDPDVTNGCSVIARIELQAGQSPTPAFLFRAGEGVGTVTLPGLGL
EIGGPAINATPRRLITGELTTILEENGLSGKIKEVVVTISVPGGKELAARTFNPKLGIIG
GISIIGTSGIVRPFSNDAFLASIRKEAEVAKAIGCSRLVINSGAKSERFLKGYLSERLSE
ELPPQMFVHYGNFIGKTLKIAAELQFDEVVMGIMIGKAVKLAEGALDTHSKKVVMNKAFL
KELAAKAGCKPESVQKIDSLTLARELWELFSKTEQQSFFPLLLQKCREHCTPLLPNGKLS
VLLISESGKITFQA