Protein Info for HMPREF1078_RS07445 in Parabacteroides merdae CL09T00C40

Annotation: precorrin-4 C(11)-methyltransferase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 645 signal peptide" amino acids 1 to 20 (20 residues), see Phobius details PF11760: CbiG_N" amino acids 50 to 130 (81 residues), 116.7 bits, see alignment E=7.6e-38 PF11761: CbiG_mid" amino acids 135 to 253 (119 residues), 58.3 bits, see alignment E=1.5e-19 PF01890: CbiG_C" amino acids 256 to 377 (122 residues), 84.2 bits, see alignment E=2e-27 TIGR01465: precorrin-4 C11-methyltransferase" amino acids 400 to 644 (245 residues), 341.7 bits, see alignment E=1.1e-106 PF00590: TP_methylase" amino acids 402 to 604 (203 residues), 154 bits, see alignment E=1.1e-48

Best Hits

Predicted SEED Role

"Cobalamin biosynthesis protein CbiG / Cobalt-precorrin-4 C11-methyltransferase (EC 2.1.1.133)" in subsystem Cobalamin synthesis or Coenzyme B12 biosynthesis (EC 2.1.1.133)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.1.1.133

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (645 amino acids)

>HMPREF1078_RS07445 precorrin-4 C(11)-methyltransferase (Parabacteroides merdae CL09T00C40)
MSKNTLNILVVSASSLSLARKIRDEYPGKTVIYCREADNDIEPVTSFKELTGNLFDKSET
IVFIGAMGICVRSIAPYIKSKYTDPAVICLDSTGKYVVSVLSGHIGGANELTHELARILD
AEAVVTTQSDNQGLWALDTFAKQYGWEQDVYNPQEGIISFFQTDFEKIDEETFNASREKA
MNECVARFVNCRPTALLLDIRTKETDYMKETCPPHVDVFYKYKDIDLEKYELLITVSPTY
YHKSPIPCLSFFPKVLHLGIGCKKGLTDMKSVLTDLYICSIFYRFNLKSIADVSSIDLKK
EEPILKELADTYLRSPFKTYPAEVLDKIPVPHPSSTVKKATGSGSVAEAAAILSAEGGPL
LVGKQKGQTKDFTYAIAISKSAIQDEEDSQQKGKQGHGHIEIVGAGPGDPELISIRGRRM
LENADLILYAGSLVPKELTLCAKKGSTIRSSADMNLEEQFALIKKFYDKGKFIVRLHTGD
PCIYGAIQEQMAFFDRYGMSYHITPGISSFQAAAAALRSQFTIPEKVQTIILTRGEGRTP
MPEKEQLHKLAASQSTMCIFLSAGIAGQVQKELLEHYPPTTPVAACYKLTWKDERIYRGE
LKDLEQIVKENNLTLTTLLVVGDAIDNRTGVSRLYAEEFKHLYRR