Protein Info for HMPREF1078_RS07330 in Parabacteroides merdae CL09T00C40

Annotation: O-acetylhomoserine aminocarboxypropyltransferase/cysteine synthase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR01326: O-acetylhomoserine aminocarboxypropyltransferase/cysteine synthase
  • amino acids 7 to 427 (421 residues), 620.2 bits, E=7.9e-191
• PF01053: Cys_Met_Meta_PP
  • amino acids 9 to 426 (418 residues), 449.7 bits, E=1.3e-138
• PF00155: Aminotran_1_2
  • amino acids 63 to 226 (164 residues), 33.7 bits, E=5e-12
• PF01041: DegT_DnrJ_EryC1
  • amino acids 68 to 202 (135 residues), 36.9 bits, E=5.3e-13
• PF00266: Aminotran_5
  • amino acids 90 to 216 (127 residues), 23 bits, E=7.4e-09

Best Hits

Swiss-Prot: 53% identical to CYSD_SCHPO: Homocysteine synthase (met17) from Schizosaccharomyces pombe (strain 972 / ATCC 24843)

KEGG orthology group: K01740, O-acetylhomoserine (thiol)-lyase [EC: 2.5.1.49] (inferred from 91% identity to pdi:BDI_2552)

MetaCyc: 52% identical to O-acetylhomoserine sulfhydrylase (Leptospira meyeri)
O-acetylhomoserine aminocarboxypropyltransferase. [EC: 2.5.1.49]

Predicted SEED Role

"O-acetylhomoserine sulfhydrylase (EC 2.5.1.49) / O-succinylhomoserine sulfhydrylase (EC 2.5.1.48)" in subsystem Methionine Biosynthesis (EC 2.5.1.48, EC 2.5.1.49)

MetaCyc Pathways

• superpathway of L-lysine, L-threonine and L-methionine biosynthesis II (12/15 steps found)
• aspartate superpathway (19/25 steps found)
• L-homocysteine biosynthesis (2/2 steps found)
• superpathway of S-adenosyl-L-methionine biosynthesis (7/9 steps found)
• superpathway of L-methionine biosynthesis (transsulfuration) (7/9 steps found)
• superpathway of L-methionine biosynthesis (by sulfhydrylation) (9/12 steps found)
• superpathway of L-homoserine and L-methionine biosynthesis (6/8 steps found)
• L-methionine biosynthesis III (3/4 steps found)
• L-methionine biosynthesis I (3/5 steps found)
• superpathway of L-lysine, L-threonine and L-methionine biosynthesis I (12/18 steps found)
• L-methionine biosynthesis II (3/6 steps found)
• superpathway of L-cysteine biosynthesis (fungi) (3/6 steps found)
• S-methyl-5-thio-α-D-ribose 1-phosphate degradation II (2/5 steps found)
• S-methyl-5-thio-α-D-ribose 1-phosphate degradation III (2/5 steps found)
• homocysteine and cysteine interconversion (1/4 steps found)
• superpathway of sulfur amino acid biosynthesis (Saccharomyces cerevisiae) (5/10 steps found)

KEGG Metabolic Maps

• Biosynthesis of plant hormones
• Cysteine metabolism
• Methionine metabolism
• Selenoamino acid metabolism
• Sulfur metabolism

Isozymes

Compare fitness of predicted isozymes for: 2.5.1.48, 2.5.1.49

Use Curated BLAST to search for 2.5.1.48 or 2.5.1.49

Protein Sequence (429 amino acids)

>HMPREF1078_RS07330 O-acetylhomoserine aminocarboxypropyltransferase/cysteine synthase (Parabacteroides merdae CL09T00C40)
MATKKLHFETLQVHVGQEQADPATDARAVPIYQTTSYVFHDSQHAADRFGLRDAGNIYGR
LTNSTQGVFEARVAALEGGVAGLAVASGAAAVTYALQNILGVGDHIVAADNLYGGSFNLI
THTLAAQGITHTIVNVNDLQALEAAIRENTKAVYVETFGNPNSDVTNIDAVAEVAHRHNI
PLIVDNTFGTPYLIRPIEHGADIVVHSATKFIGGHGSSLGGVIVDSGNFDWKANSDKFPT
LGTPDPSYHGAVFADVAGSAAFVTRIRAVILRDTGATISPFNAFILLQGLETLSLRVERH
VANALKVVEYLSKHPKVTKVNHPALPDHPDHELYKKYFPKGAGSIFTFEIEGGQVAAWKF
IDSLEIFSLLANVADVKSLVIHPYTTTHSQMTPEELAGQHITPSIVRLSIGTEYIDDIID
DLDQALAKV