Protein Info for HMPREF1078_RS06765 in Parabacteroides merdae CL09T00C40

Annotation: tyrosine--tRNA ligase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 430 TIGR00234: tyrosine--tRNA ligase" amino acids 2 to 428 (427 residues), 407.4 bits, see alignment E=3.7e-126 PF00579: tRNA-synt_1b" amino acids 27 to 325 (299 residues), 276.8 bits, see alignment E=1.1e-86

Best Hits

Swiss-Prot: 90% identical to SYY_PARD8: Tyrosine--tRNA ligase (tyrS) from Parabacteroides distasonis (strain ATCC 8503 / DSM 20701 / CIP 104284 / JCM 5825 / NCTC 11152)

KEGG orthology group: K01866, tyrosyl-tRNA synthetase [EC: 6.1.1.1] (inferred from 90% identity to pdi:BDI_3058)

MetaCyc: 51% identical to tyrosine--tRNA ligase (Escherichia coli K-12 substr. MG1655)
Tyrosine--tRNA ligase. [EC: 6.1.1.1]; 6.1.1.1 [EC: 6.1.1.1]

Predicted SEED Role

"Tyrosyl-tRNA synthetase (EC 6.1.1.1)" (EC 6.1.1.1)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 6.1.1.1

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (430 amino acids)

>HMPREF1078_RS06765 tyrosine--tRNA ligase (Parabacteroides merdae CL09T00C40)
MNFVEELTWRGMIADMMPGTEEQLQKEMTSAYVGIDPTADSLHIGHLVSVMMLKHLQRAG
HRPIALVGGATGMIGDPSMKSAERNLLDEATLRHNQDSIKKQLSKFLDFDSDAPNAAKLV
NNYDWMKDYSFLNFIRDIGKHITVNYMMAKESVKKRLSRESSVGMSFTEFSYQLLQGYDY
MYLYEHEGCRLQMGGTDQWGNITTGTELIRRKLGGEAFALTCPLITKADGGKFGKTESGN
VWLDRRYTSPYKFYQFWLNVSDADAAKYIKIFTDLPKEEIDALIKEQEEAPHLRPLQKRL
AKEVTVMVHSQEDYDAAVEASNILFGNSTSEALKHLDEQTLLDVFNGVPQFEVSREELSA
GVKAIDLFTEKAAIFPSKGEMRKLVQSGGISVNKEKLTDQDMVIDCSSLLDEKYLLVQRG
KKNYYLLIVK