Protein Info for HMPREF1078_RS06225 in Parabacteroides merdae CL09T00C40

Annotation: exo-alpha-sialidase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 539 signal peptide" amino acids 1 to 20 (20 residues), see Phobius details PF14873: BNR_assoc_N" amino acids 20 to 167 (148 residues), 157.6 bits, see alignment E=5e-50 PF13859: BNR_3" amino acids 200 to 492 (293 residues), 112.2 bits, see alignment E=8.9e-36 PF13088: BNR_2" amino acids 229 to 506 (278 residues), 89.8 bits, see alignment E=4.8e-29

Best Hits

Swiss-Prot: 65% identical to NANH_BACFR: Sialidase (nanH) from Bacteroides fragilis (strain YCH46)

KEGG orthology group: K01186, sialidase-1 [EC: 3.2.1.18] (inferred from 78% identity to pdi:BDI_2946)

Predicted SEED Role

"Sialidase (EC 3.2.1.18)" in subsystem Galactosylceramide and Sulfatide metabolism or Sialic Acid Metabolism (EC 3.2.1.18)

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 3.2.1.18

Use Curated BLAST to search for 3.2.1.18

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (539 amino acids)

>HMPREF1078_RS06225 exo-alpha-sialidase (Parabacteroides merdae CL09T00C40)
MRKIFLYFSLLLFMQTAFAADSLFVRKPQIPILIDRTDNILVEMRVQAHKGDVLNKLSLQ
FKEGIDLNDIKALRFFYSGTEATSRQGKHYRPVSYISSHAEGKTKAANPAYSIKQSEVTD
ITNVVTFTSNQPMVEGVNYYWISIEMKPEASLLTTFTVQMPMAEINNMPATIVWDGKSDV
RRMGIGVRHAGDDGASAYRIPGLVTTNNGTLLGVYDIRYNSSVDLQEMVDIGVSRSTDKG
QTWEPMRVAMTFGETGGLPHAQNGVGDPSILVDEKTNTIWIVAAWTHGMGNGRAWWNSMP
GMSADSTAQLMLVKSEDDGKTWSQPINITPQVKDPSWYFLLQGPGRGITMKDGTLVFPIQ
FIDSTRIPNAGIMYSKDRGTTWHLHNLARTNTTEAQVAEIEPGVLMLNMRDNRGGSRAVA
TTRDLGKTWYEHPSSRSALQEPVCMASLIHVDAKDNITGKDLLIFSNPNTTKGRNHITIK
VSTDGGMTWPLSNQVTLDEDESWGYSCLSMIDKETVGIFYESSVAHMTFQAVKLTDLVK