Protein Info for HMPREF1078_RS05865 in Parabacteroides merdae CL09T00C40

Annotation: glycoside hydrolase family 2 TIM barrel-domain containing protein

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 833 signal peptide" amino acids 1 to 18 (18 residues), see Phobius details PF02837: Glyco_hydro_2_N" amino acids 27 to 179 (153 residues), 100.8 bits, see alignment E=2e-32 PF00703: Glyco_hydro_2" amino acids 189 to 292 (104 residues), 56.5 bits, see alignment E=1.1e-18 PF02836: Glyco_hydro_2_C" amino acids 302 to 513 (212 residues), 90.9 bits, see alignment E=2.1e-29 PF16355: DUF4982" amino acids 654 to 712 (59 residues), 72.9 bits, see alignment 4.1e-24 PF18565: Glyco_hydro2_C5" amino acids 726 to 827 (102 residues), 117.1 bits, see alignment E=7.5e-38

Best Hits

Predicted SEED Role

"Beta-galactosidase (EC 3.2.1.23)" in subsystem Galactosylceramide and Sulfatide metabolism or Lactose and Galactose Uptake and Utilization or Lactose utilization (EC 3.2.1.23)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 3.2.1.23

Use Curated BLAST to search for 3.2.1.23

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (833 amino acids)

>HMPREF1078_RS05865 glycoside hydrolase family 2 TIM barrel-domain containing protein (Parabacteroides merdae CL09T00C40)
MRLSVFALVCLFLTACATREKGRERISFNKEWRFALTEKQANASAPDTDDSNWRVLNLPH
DWSIEADFSLDNPATPGGGALPGGMGWYRKHFKLSESDKGKVIYIDFDGVYRNSRVWLNG
HLLGFRPNGYVSFRYDLTPYLNYGKEENVLVVQADNSDQPNSRWYSGSGIYRNVWLVKTG
QVHVDNWGTNITTPVMNADKSTVEIETTLKNEASDVTVEVSTLIQDKEGKTLYRISSPAE
IAAGGKKIVTHTIEMFYPELWSVRNPYLYTAITEVRVGNKLTDRYRTTFGIRKFDWDEHT
GFSLNGEPTKILGVCLHHDLGCLGSAVNPRALERQLQMMKDMGANAVRTSHNPPAPELLD
ICDRIGLLVQDEAFDMWRKRKSPYDYAQYFDEWHEKDLTDQVLRDRNHPSVFMWSIGNEV
LEQWQHADADTLSLEAANLILNAGHPVDDKILSDTAMSVQGLIAHSLAAIVKRLDKTRPV
TAANNEATPGNLIFRSNALDVLGFNYHEKNYEPFPQNFPGKTLIVSESTSALMTRGYYQM
PSDSMYVWPNTWRERFDRPEHLCSAYDNCHVPWGSTHEVTWREVKRLPYVSGMFIWTGFD
YLGEPTPYWWPSRSSFFGIVDLAGIPKDVYYMYQSEWTDTPVLHLFPHWNWKKGETVDVW
AYYNRADEVELFLNGESLGVKSKPDDAFHVCWRVPFTPGTLKAVSRRDGKEVLTREIRTA
GEPARIMLIPDRSALHADGTDLSFVTVEIQDKDGNLVPYADNLLRFTVEGDGFIAGTDNG
DQNDPVSLKKPERHAFYGKAMAVIQNTGKAGTIRLKATAEGLPDAIVEIKVGE