Protein Info for HMPREF1078_RS04420 in Parabacteroides merdae CL09T00C40

Annotation: RelA/SpoT family protein

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 764 PF13328: HD_4" amino acids 56 to 206 (151 residues), 147.9 bits, see alignment E=5.3e-47 TIGR00691: RelA/SpoT family protein" amino acids 56 to 762 (707 residues), 654.3 bits, see alignment E=1.1e-200 PF01966: HD" amino acids 76 to 175 (100 residues), 31.3 bits, see alignment E=5.7e-11 PF04607: RelA_SpoT" amino acids 267 to 382 (116 residues), 127 bits, see alignment E=1e-40 PF02824: TGS" amino acids 423 to 482 (60 residues), 77.6 bits, see alignment 1.5e-25 PF13291: ACT_4" amino acids 685 to 761 (77 residues), 59.3 bits, see alignment E=1.2e-19

Best Hits

KEGG orthology group: K00951, GTP pyrophosphokinase [EC: 2.7.6.5] (inferred from 87% identity to pdi:BDI_2550)

Predicted SEED Role

"GTP pyrophosphokinase (EC 2.7.6.5) / Guanosine-3',5'-bis(diphosphate) 3'-pyrophosphohydrolase (EC 3.1.7.2)" in subsystem Stringent Response, (p)ppGpp metabolism (EC 2.7.6.5, EC 3.1.7.2)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 2.7.6.5

Use Curated BLAST to search for 2.7.6.5 or 3.1.7.2

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (764 amino acids)

>HMPREF1078_RS04420 RelA/SpoT family protein (Parabacteroides merdae CL09T00C40)
MSDINDTKDTAGMQPDQKAKPALSADEQMIQDGFNALLQDYLNSNHRRKVERITKAFNFA
NQAHAGVKRRSGEPYIMHPIAVARIVCREMGLGSTSICSALLHDVVEDTEYTVEDIKDMF
GEKIAQIVDGLTKISGGIFGEQASAQAENFRKLLLTMSDDIRVILIKIADRLHNMRTLGS
MLPAKQFKIAGETLYLYAPLAHRLGLFTIKTELEDLSFKYEHPQEYAFINLKLKSTEAAR
NTLFEHFAVPVDRKLKEMGLNYEMRARVKSAYSIWNKMESKGVSFEDIYDIYAVRIIFDP
LPGVDEKNQCWDIYSAITDIYRIRPDRIRDWVSRPKANGYQALHLTVMGPDGQWVEIQIR
SRRMDEIAEKGFAAHWKYKEHNIEEDTELDKWLQTITEILESPDPNALDFLDTIKLNLFT
SEIFVFTPKGDIKTLPQGATALDFGYALHSDIGNKCIGAKVNHRLVPLSHQLASGDQVEI
LTSRSQNPQPEWLNFVTTARARARIDAYLKRIRKEAAKEGEIKVIDAFKRNNLEANTSNI
DKLCMYFGFSKREEFYYAVEKGEVILPENIHKLLKEKTDNVLFKYVKQALRVATKATTGK
KEEEEQPKEKPKYDKKKPYILKEEAFERNYVIADCCKPIPGDESLGFINDDGNVVVHKRS
CPIAMRLKSSFGERILNTEWSSHHSSFEATLEIKGIDSIGVLNTITRTISDDFNVNIIRL
LIEAKDGVFEGKVKMKVHDVEDIQRMCVVLSKIKNIQSVARVAD