Protein Info for HMPREF1078_RS02440 in Parabacteroides merdae CL09T00C40

Annotation: bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 375 TIGR01662: HAD hydrolase, family IIIA" amino acids 3 to 149 (147 residues), 58.3 bits, see alignment E=1.4e-19 TIGR01261: histidinol-phosphatase" amino acids 3 to 153 (151 residues), 175.7 bits, see alignment E=7.3e-56 TIGR01656: histidinol-phosphate phosphatase domain" amino acids 4 to 147 (144 residues), 98.5 bits, see alignment E=5.2e-32 PF13242: Hydrolase_like" amino acids 104 to 159 (56 residues), 27.6 bits, see alignment 2.3e-10 PF00475: IGPD" amino acids 214 to 355 (142 residues), 207.8 bits, see alignment E=7.8e-66

Best Hits

KEGG orthology group: K01089, imidazoleglycerol-phosphate dehydratase / histidinol-phosphatase [EC: 3.1.3.15 4.2.1.19] (inferred from 86% identity to pdi:BDI_0969)

Predicted SEED Role

"Histidinol-phosphatase (EC 3.1.3.15) / Imidazoleglycerol-phosphate dehydratase (EC 4.2.1.19)" in subsystem Histidine Biosynthesis (EC 3.1.3.15, EC 4.2.1.19)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 3.1.3.15

Use Curated BLAST to search for 3.1.3.15 or 4.2.1.19

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (375 amino acids)

>HMPREF1078_RS02440 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB (Parabacteroides merdae CL09T00C40)
MKRALFIDRDGTLVIEPPVDYQLDSLEKLVFYPKVFRNLYFIRKQLDFEFVMVTNQDGLG
TDSFPEDTFWPAHDKMLKTLEGEGIRFDDILIDRSFPEENSPNRKPRTGMLGRYLSGEYD
LANSYVIGDRLTDMQLAANLGAKGIWLRPDDVEARQLLTENTAISPVLITDDWDRITEYL
FAGERRGTIRRTTKETDIFVEVNLDGHGRTEISTGLGFFDHMLDQIGKHSGIDLTVRVKG
DLEVDEHHTIEDTAIALGEALLKALGDKRGIERYGYCLPMDDCLCSVALDFGGRPWLVWD
AAFHREKVGDMPTEMFLHFFKSLSDAARMNLNIKAEGTNEHHKIEGIFKALARSIKMAIR
RDIYRYELPSTKGAL