Protein Info for HMPREF1078_RS01935 in Parabacteroides merdae CL09T00C40

Annotation: alpha-ketoacid dehydrogenase subunit alpha/beta

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 678 PF00676: E1_dh" amino acids 40 to 320 (281 residues), 194.5 bits, see alignment E=4.4e-61 PF02779: Transket_pyr" amino acids 350 to 528 (179 residues), 84.1 bits, see alignment E=1.9e-27 PF02780: Transketolase_C" amino acids 545 to 668 (124 residues), 119.1 bits, see alignment E=2.3e-38

Best Hits

KEGG orthology group: K11381, 2-oxoisovalerate dehydrogenase E1 component [EC: 1.2.4.4] (inferred from 85% identity to bth:BT_0312)

Predicted SEED Role

"Acetoin dehydrogenase E1 component alpha-subunit (EC 1.2.4.-) / Acetoin dehydrogenase E1 component beta-subunit (EC 1.2.4.-)" in subsystem Acetoin, butanediol metabolism (EC 1.2.4.-)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 1.2.4.- or 1.2.4.4

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (678 amino acids)

>HMPREF1078_RS01935 alpha-ketoacid dehydrogenase subunit alpha/beta (Parabacteroides merdae CL09T00C40)
MKRYDIKTTDTETLRKWYYLMSLGRALDEKAPSYLLQSLGWSYHAPYAGHDGIQLAIGQV
FHQGEDYLFPYYRDMLTVLSAGMTAEEIILNGISKATDPSSGGRHMSNHFAKPEWHIENI
SSATGTHDLHAVGVARAMVYYDHKGVAITSHGESATSEGYVYEAINGASNEQLPVIFVLQ
DNGYGISVPKKDQTANRKVADNFSGFKNLRIIHCNGKDVFDSMNAMTEAREHAINTHNPV
IVHANCVRIGSHSNSDKQTLYRDENELAYVKAADPLQKFRRMLLRYNRLTEEELKQIEAQ
VKKDLSAANRKAMAAPEPDPATIFDYVLPEPYLPQKYTDGTHKEENGEKKTLVTAINETL
KAEFRHNPNTFLWGQDVANKDKGGVFNVTKGMQQEFGPKRIFNAPIAEDYIVGTANGMCR
FDPKIHVVVEGAEFADYFWPAIEQYVECTHEYWRSNGQFTPNLTLRLASGGYIGGGLYHS
QTIEGALTSIPGARIVYPSFADDAAGLLRTSIRSKGFTVFLEPKAQYNSVEAAGFVPEDF
EVPFGKARIRRPGNDLSVITYGNTTHFCLSVAEKLKKEHNWDVEVIDIRSLIPLDTETIF
ASVKKTGKVLIVHEDKVFSGFGAEIAGIVGTEMFRYLDAPIQRVGSTFTPVGFHPVLEKA
ILPGEERIYNAAKALLEY