Protein Info for HMPREF1078_RS00885 in Parabacteroides merdae CL09T00C40

Annotation: sulfide-dependent adenosine diphosphate thiazole synthase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 256 transmembrane" amino acids 21 to 42 (22 residues), see Phobius details TIGR00292: thiazole biosynthesis enzyme" amino acids 4 to 254 (251 residues), 291.8 bits, see alignment E=1.9e-91 PF01946: Thi4" amino acids 8 to 236 (229 residues), 242.3 bits, see alignment E=1.9e-75 PF01494: FAD_binding_3" amino acids 23 to 54 (32 residues), 24.5 bits, see alignment 6.8e-09 PF07992: Pyr_redox_2" amino acids 24 to 63 (40 residues), 26.7 bits, see alignment 1.7e-09 PF01266: DAO" amino acids 24 to 62 (39 residues), 34 bits, see alignment 1.1e-11 PF01134: GIDA" amino acids 24 to 55 (32 residues), 22.1 bits, see alignment 3.3e-08 PF12831: FAD_oxidored" amino acids 24 to 141 (118 residues), 35.4 bits, see alignment E=3.7e-12 PF00890: FAD_binding_2" amino acids 24 to 54 (31 residues), 22.4 bits, see alignment 3.1e-08 PF13450: NAD_binding_8" amino acids 27 to 59 (33 residues), 34.8 bits, see alignment 7.2e-12

Best Hits

Swiss-Prot: 53% identical to THI4_METTH: Thiamine thiazole synthase (thi4) from Methanothermobacter thermautotrophicus (strain ATCC 29096 / DSM 1053 / JCM 10044 / NBRC 100330 / Delta H)

KEGG orthology group: K03146, thiamine biosynthetic enzyme (inferred from 94% identity to pdi:BDI_1609)

MetaCyc: 49% identical to sulfide-dependent adenosine diphosphate thiazole synthase (Methanocaldococcus jannaschii)
RXN-19351 [EC: 2.4.2.59]

Predicted SEED Role

"Thiazole biosynthetic enzyme Thi4 / Ribose 1,5-bisphosphate or 5-ribose-1,2-cyclic phosphate dehydrogenase" in subsystem Calvin-Benson cycle or Thiamin biosynthesis

MetaCyc Pathways

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.4.2.59

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (256 amino acids)

>HMPREF1078_RS00885 sulfide-dependent adenosine diphosphate thiazole synthase (Parabacteroides merdae CL09T00C40)
MEQIVSTGIIDSYFAKLKSNLSIDVAIVGGGPSGIVAAYYLAKAGKKVALFDRKLAPGGG
MWGGAMMFNDIVVQEEAMPIVRELGVSYKDAGNGTYIMDSVHTTSALIYGATKAGATIFN
CYSVEDVVFHNDAVAGVVVNWAPVIREGMHVDPLTIMSKAVLEGTGHDCEIARVVARKND
IKLNTPTGGVIGERSLNVELGESTTVENTKEIYPGLFVSGMAANGVSGSFRMGPIFGGML
MSGKKAAELICAKLDK