Protein Info for HMPREF1058_RS16775 in Phocaeicola vulgatus CL09T03C04

Annotation: tRNA pseudouridine(38-40) synthase TruA

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 257 TIGR00071: tRNA pseudouridine(38-40) synthase" amino acids 6 to 236 (231 residues), 180.2 bits, see alignment E=2.5e-57 PF01416: PseudoU_synth_1" amino acids 8 to 103 (96 residues), 44 bits, see alignment E=1.3e-15 amino acids 141 to 243 (103 residues), 83.3 bits, see alignment E=8.5e-28

Best Hits

Swiss-Prot: 75% identical to TRUA_BACTN: tRNA pseudouridine synthase A (truA) from Bacteroides thetaiotaomicron (strain ATCC 29148 / DSM 2079 / NCTC 10582 / E50 / VPI-5482)

KEGG orthology group: K06173, tRNA pseudouridine synthase A [EC: 5.4.99.12] (inferred from 99% identity to bvu:BVU_2546)

Predicted SEED Role

"tRNA pseudouridine synthase A (EC 4.2.1.70)" in subsystem tRNA processing (EC 4.2.1.70)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 4.2.1.70, 5.4.99.12

Use Curated BLAST to search for 4.2.1.70 or 5.4.99.12

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See I9A4G6 at UniProt or InterPro

Protein Sequence (257 amino acids)

>HMPREF1058_RS16775 tRNA pseudouridine(38-40) synthase TruA (Phocaeicola vulgatus CL09T03C04)
MRYFIYLAYDGTNYHGWQIQPNGDSVQETLMRALSTFLRQEIEVVGAGRTDAGVHARLMI
AHFDYDRELDCVQITEKLNRLLPPDIAVYAVKRVKDDAHARFDATYRTYKYYVTTRKDPF
NRHYAWRLFNLLDFEKMNEAARVLFDYTDFTSFSKLHTDVKTNNCRIMHAEWTQVSGTEW
VFTIKADRFLRNMVRAVVGTLVEVGRGKMTVDEFRKVIEDKDRCSAGSSVPGHALFLVDV
GYPEELFIVSPSDISNI