Protein Info for HMPREF1058_RS16685 in Phocaeicola vulgatus CL09T03C04

Annotation: methionine synthase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR02082: methionine synthase
  • amino acids 9 to 911 (903 residues), 1336.6 bits, E=0
• PF02574: S-methyl_trans
  • amino acids 13 to 319 (307 residues), 337.1 bits, E=2.5e-104
• PF00809: Pterin_bind
  • amino acids 360 to 598 (239 residues), 205.2 bits, E=2.7e-64
• PF02607: B12-binding_2
  • amino acids 660 to 732 (73 residues), 81.1 bits, E=1.1e-26
• PF02310: B12-binding
  • amino acids 748 to 840 (93 residues), 73.3 bits, E=3.1e-24

Best Hits

KEGG orthology group: K00548, 5-methyltetrahydrofolate--homocysteine methyltransferase [EC: 2.1.1.13] (inferred from 100% identity to bvu:BVU_2528)

Predicted SEED Role

"5-methyltetrahydrofolate--homocysteine methyltransferase (EC 2.1.1.13)" in subsystem Methionine Biosynthesis (EC 2.1.1.13)

MetaCyc Pathways

• folate transformations III (E. coli) (8/9 steps found)
• folate transformations II (plants) (9/11 steps found)
• aspartate superpathway (19/25 steps found)
• superpathway of S-adenosyl-L-methionine biosynthesis (7/9 steps found)
• superpathway of L-methionine biosynthesis (transsulfuration) (7/9 steps found)
• superpathway of L-homoserine and L-methionine biosynthesis (6/8 steps found)
• folate transformations I (9/13 steps found)
• L-methionine biosynthesis I (3/5 steps found)
• superpathway of L-methionine biosynthesis (by sulfhydrylation) (8/12 steps found)
• L-methionine biosynthesis III (2/4 steps found)
• superpathway of L-lysine, L-threonine and L-methionine biosynthesis I (12/18 steps found)
• L-methionine salvage from L-homocysteine (1/3 steps found)
• superpathway of L-methionine salvage and degradation (7/16 steps found)

KEGG Metabolic Maps

• Methionine metabolism
• One carbon pool by folate

Isozymes

Compare fitness of predicted isozymes for: 2.1.1.13

Use Curated BLAST to search for 2.1.1.13

Sequence Analysis Tools

See I9UK03 at UniProt or InterPro

Protein Sequence (917 amino acids)

>HMPREF1058_RS16685 methionine synthase (Phocaeicola vulgatus CL09T03C04)
MATLKQLIDERVLILDGAMGTMIQRYNLSEQDFRGERFAEMPGQMKGNNDLLCLTRPDVI
KDIHHKYLEAGADIIETNTFNAQRVSMADYHMQDLCREINLAAAGLAREMADEYTAKTPH
KPRFVAGSVGPTNKTCSMSPDVNNPALRALTYDELAAAYQEQMEALLEGGVDALLIETIF
DSLNAKAAIYAAETAMKKIGREVPLMLSVTVSDIAGRTLSGQTLDAFLASVQHAPIFSIG
LNCSFGAKQLKPFLEGLAARAPYYISAYPNAGLPNSLGQYDQTPEEMASEVKEYIDEGLV
NIIGGCCGTTEEYIAKYQELIVSGSAWVSPHIPATTPERLWLSGLELLEQTPEMNFINVG
ERCNVAGSRKFLRLINEKKYEEALSIARKQVEDGALVIDVNMDDGLLDAREEMTTFLNLV
MSEPDIARVPVMIDSSKWEVIEAGLKCLQGKSIVNSISLKEGEEIFIEHARLIKKLGAAV
VVMAFDEKGQADTFERKIEVCARAYKILTEQVDFNPHDIIFDPNVLAVATGIEEHDNYAV
DFIKATGWIKKNLPGAHVSGGVSNLSFSFRGNNYIREAMHAVFLYHAIRQGMDMGIVNPA
ASVLYTDIPADVLERIEDVVLNRRPDAAERLIETAEALKRTSTGTEAVKQDVWREEPMVE
KRLQYALIKGIGDHLEEDLAEAVKLYPKAVDIIEGPLMEGMNKVGELFGAGKMFLPQVVK
TARTMKKAVAILQPLIEADKQEGVRSAGKVLMATVKGDVHDIGKNIVSVVMACNNYEIID
LGVMVPAEMIVRKAIEEKVDIIGLSGLITPSLEEMAHVAVELKRAGLDIPIMIGGATTSK
LHTALKIAPVYGGPVIHMKDASQNALVAARLLNPESSFEFVERLNKEYEDLRLKNSAKQV
KTVSLEEAQKNKLNLWS