Protein Info for HMPREF1058_RS15675 in Phocaeicola vulgatus CL09T03C04

Annotation: bifunctional folylpolyglutamate synthase/dihydrofolate synthase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR01499: bifunctional protein FolC
  • amino acids 31 to 426 (396 residues), 387.8 bits, E=4e-120
• PF08245: Mur_ligase_M
  • amino acids 51 to 239 (189 residues), 44.2 bits, E=1.1e-15

Best Hits

KEGG orthology group: K11754, dihydrofolate synthase / folylpolyglutamate synthase [EC: 6.3.2.12 6.3.2.17] (inferred from 100% identity to bvu:BVU_2328)

Predicted SEED Role

"Dihydrofolate synthase (EC 6.3.2.12) / Folylpolyglutamate synthase (EC 6.3.2.17)" in subsystem Folate Biosynthesis (EC 6.3.2.12, EC 6.3.2.17)

MetaCyc Pathways

• folate polyglutamylation (5/5 steps found)
• superpathway of tetrahydrofolate biosynthesis and salvage (10/12 steps found)
• tetrahydrofolate biosynthesis I (3/3 steps found)
• superpathway of tetrahydrofolate biosynthesis (8/10 steps found)
• tetrahydrofolate biosynthesis II (2/3 steps found)
• superpathway of chorismate metabolism (38/59 steps found)

KEGG Metabolic Maps

• Biosynthesis of phenylpropanoids
• Folate biosynthesis

Isozymes

No predicted isozymes

Use Curated BLAST to search for 6.3.2.12 or 6.3.2.17

Sequence Analysis Tools

See I9UHE0 at UniProt or InterPro

Protein Sequence (428 amino acids)

>HMPREF1058_RS15675 bifunctional folylpolyglutamate synthase/dihydrofolate synthase (Phocaeicola vulgatus CL09T03C04)
MNYEETLDYLYNSAPLFQHIGKDAYKAGLENTYLLDKYFNHPHRQFRTIHIAGTNGKGSC
SHTLAAILQSAGYKTGLYTSPHLIDFRERIRVNGIPVSKEYVIDFVEKHRAFFEPLHPSF
FELTTAMAFHYFAQSQVDVAIIEVGLGGRIDCTNIIRPDLCVITNISFDHIQFLGNTLAK
IATEKAGIIKEKTPVVIGETTPETKPIFTTRAKEVNAPIYFAEEEQLLHSSSINEKGKRI
YQTTDYLNLEGELEGLCQLKNTNTLLSAIRLLKQAGYQLTESNIRKGFSQVCELTGLMGR
WQKLESEPTLICDTGHNVGGISYIIEQLKHQKYERLHIVIGMVNDKDISGVLSMLPKNAT
YYFTKASVKRALSEKELQSLAMQSGLHGDTYPDVETAVTAAKEKANKNDFIFVGGSSFIV
ADLLKFHV