Protein Info for HMPREF1058_RS15105 in Phocaeicola vulgatus CL09T03C04

Annotation: glycoside hydrolase family 97 protein

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 658 signal peptide" amino acids 1 to 20 (20 residues), see Phobius details PF14508: GH97_N" amino acids 25 to 290 (266 residues), 196.3 bits, see alignment E=8.3e-62 PF10566: Glyco_hydro_97" amino acids 295 to 560 (266 residues), 331.7 bits, see alignment E=5.6e-103 PF14509: GH97_C" amino acids 562 to 657 (96 residues), 103.2 bits, see alignment E=1.2e-33

Best Hits

Swiss-Prot: 72% identical to AGAL_BACTN: Retaining alpha-galactosidase (BT_1871) from Bacteroides thetaiotaomicron (strain ATCC 29148 / DSM 2079 / NCTC 10582 / E50 / VPI-5482)

KEGG orthology group: None (inferred from 72% identity to bth:BT_1871)

Predicted SEED Role

"Alpha-glucosidase (EC 3.2.1.20)" in subsystem D-Galacturonate and D-Glucuronate Utilization or Maltose and Maltodextrin Utilization (EC 3.2.1.20)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 3.2.1.20

Use Curated BLAST to search for 3.2.1.20

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See I9A2W5 at UniProt or InterPro

Protein Sequence (658 amino acids)

>HMPREF1058_RS15105 glycoside hydrolase family 97 protein (Phocaeicola vulgatus CL09T03C04)
MKKLTILFLCSWIVLTAQARKVFQLDSPDGHLSTTITLGDQLTYDITCNGQQVLAASPLS
MTLEDGTVWGDHPQLAGSSRKEVDQLIDSPFYRSSRLRDHYKKLVLCFRKGWSVEFRAYN
DGIAYRFVNQMKKPFKILQEEVNYQFTTDKTAIVPYVVPRSEDQFYSAFENTYTISKISE
LDKQKLMFLPLVVEVGDGVKVCITESDLENYPGLYLSGATGGNSLTGVHAPYPASKRQGG
HNNLQMIVEKRENFIAKVDGPRTFPWRMAIVTTDDKDLAASSMTYLLSSPSRIGDLSWIK
PGKVAWEWWSDWNLDGVDFVTGVNNDTYKAYIDFAAAHQLEYVILDEGWAVNLQADLMQV
VKDINLKELVDYAAARNVGIILWAGYYAFDRDMENLCRYYAELGVKGFKVDFMDRDDQEM
VAFNYRAAETCAKYKLLLDLHGMYKPSGLNRTYPNVLNFEGVHGLENMKWSPESVDQVKY
DVTIPFIRQVAGPMDYTQGAMRNAVKGSYFPCNSEPMSQGTRCRQLALYVIFESPLNMLC
DTPGNYEREVECTDFITGIPTVWDETHILDAKLGEYIVTARRKGDSWYIGGITDGVARNL
EIDCSFLGDKDYVGDLFKDGMNAHRIGKDFKRESVRLNKRDKLKIHLAPGGGFAIRLQ