Protein Info for HMPREF1058_RS14835 in Phocaeicola vulgatus CL09T03C04

Annotation: single-stranded-DNA-specific exonuclease RecJ

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 573 TIGR00644: single-stranded-DNA-specific exonuclease RecJ" amino acids 28 to 568 (541 residues), 554.4 bits, see alignment E=1.1e-170 PF01368: DHH" amino acids 82 to 229 (148 residues), 92.8 bits, see alignment E=3e-30 PF02272: DHHA1" amino acids 321 to 443 (123 residues), 67.1 bits, see alignment E=3.5e-22 PF17768: RecJ_OB" amino acids 459 to 567 (109 residues), 88.5 bits, see alignment E=4.6e-29

Best Hits

KEGG orthology group: K07462, single-stranded-DNA-specific exonuclease [EC: 3.1.-.-] (inferred from 100% identity to bvu:BVU_2071)

Predicted SEED Role

"Single-stranded-DNA-specific exonuclease RecJ (EC 3.1.-.-)" in subsystem DNA-replication or DNA Repair Base Excision or DNA repair, bacterial RecFOR pathway (EC 3.1.-.-)

Isozymes

Compare fitness of predicted isozymes for: 3.1.-.-

Use Curated BLAST to search for 3.1.-.-

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See I9A318 at UniProt or InterPro

Protein Sequence (573 amino acids)

>HMPREF1058_RS14835 single-stranded-DNA-specific exonuclease RecJ (Phocaeicola vulgatus CL09T03C04)
MNHKWNYQPPSQEQTEAAKALAKETGISPILCKLLLERGITSAAEAKRFFRPQLNELHDP
FLMKDMSIAVERLNQAMGRKERIMVYGDYDVDGTTAVALVYKFLQQFYSNIDYYIPDRYN
EGYGVSVKGVDYACETGVKLIIVLDCGIKAVEEIAYAKEKGIDFIICDHHVPDDILPPAV
AILNAKRADNTYPYDHLSGCGVGFKFMQAFALNNGIEFHQLTPLLDLVAVSVASDIVPIM
GENRVLTHHGLKQLNSNPSVGLKAIIDVCGLSEKEITVGDIVFKIGPRINASGRIQNGKE
AVELLIEKDFSAALEKANQINQYNETRKDLDKTMTEEANQIVDHLEGLADRRSIVIYNEA
WHKGVIGIVASRLTEIYYRPAVVLTRTDNLATGSARSVSGFDVYKAIEHCRDLLENFGGH
TYAAGLSMKVENVEEFTRRFETYVTEHILPEQTSAVIDIDAEIDFRDITPKFHADLKKFN
PFGPDNHKPIFCTHNVYDYGTSKVVGRDQEHIKLELVDNKSNNVMNGIAFGQSSQARYIK
TKRSFDICYTIEENTHKRGEVQLQIEDIKPSEE