Protein Info for HMPREF1058_RS14585 in Phocaeicola vulgatus CL09T03C04

Annotation: DNA helicase RecQ

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 605 TIGR01389: ATP-dependent DNA helicase RecQ" amino acids 2 to 601 (600 residues), 744.1 bits, see alignment E=1.1e-227 TIGR00614: ATP-dependent DNA helicase, RecQ family" amino acids 4 to 450 (447 residues), 567.7 bits, see alignment E=2e-174 PF04851: ResIII" amino acids 14 to 171 (158 residues), 29.5 bits, see alignment E=2e-10 PF00270: DEAD" amino acids 15 to 173 (159 residues), 92.7 bits, see alignment E=6.9e-30 PF00271: Helicase_C" amino acids 213 to 321 (109 residues), 78.9 bits, see alignment E=1e-25 PF16124: RecQ_Zn_bind" amino acids 333 to 391 (59 residues), 64.4 bits, see alignment 3.9e-21 PF09382: RQC" amino acids 393 to 503 (111 residues), 100.4 bits, see alignment E=1.8e-32 PF00570: HRDC" amino acids 533 to 599 (67 residues), 72.4 bits, see alignment E=7e-24

Best Hits

Swiss-Prot: 47% identical to RECQ_HAEIN: ATP-dependent DNA helicase RecQ (recQ) from Haemophilus influenzae (strain ATCC 51907 / DSM 11121 / KW20 / Rd)

KEGG orthology group: K03654, ATP-dependent DNA helicase RecQ [EC: 3.6.4.12] (inferred from 100% identity to bvu:BVU_2013)

Predicted SEED Role

"ATP-dependent DNA helicase RecQ" in subsystem DNA-replication or DNA repair, bacterial RecFOR pathway

Isozymes

Compare fitness of predicted isozymes for: 3.6.4.12

Use Curated BLAST to search for 3.6.4.12

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See I9A4B2 at UniProt or InterPro

Protein Sequence (605 amino acids)

>HMPREF1058_RS14585 DNA helicase RecQ (Phocaeicola vulgatus CL09T03C04)
MFQTLKNYFGYDSFRPLQQDIIQNILAQKDTLVLMPTGGGKSICYQLPALLMEGTAIVVS
PLISLMKDQVESLQANGIAARALNSSNNETENINLRRECLQGKIKLLYISPERLLIETNF
LLKDIQISLFAIDEAHCISQWGHDFRPEYTQLKVLRNQFPKVPIVALTATADKITRKDIV
QQLALKDPKIFISSFDRPNLSLEVKRGYQQKDKMRTILEFIEKHKNECGIIYCMSRSKTE
NVAAMLMKQGIRATVYHAGLSSDMRDKAQNDFINDRVQVVCATIAFGMGIDKSNVRWVIH
YNLPKSIESFYQEIGRAGRDGMESDTLLFYSLGDLVMLSKFATESSQQEINLEKLHRMQQ
YAESDICRRRILLNYFGETMDHDCGNCDVCRNPPERFDGTIIVQKALSAIARTNQQIGTH
MLIDILKGSANQELIDKGYDKLKTYAAGRDIPARDWQDYLLQMLNLGYFEIAYNENNHLK
ITESGQKVLFGKESAMLVVIKREEFVPAKEKRKKKAKEEVLFPTPVTFGNENKDLFEELR
ILRKKLADQQAIPAYIVLSDKTLHLIATQQPTTIEAFGNINGIGEYKKEKYGKEFIELIK
KVLKK