Protein Info for HMPREF1058_RS14395 in Phocaeicola vulgatus CL09T03C04

Annotation: HAD family hydrolase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 217 PF00702: Hydrolase" amino acids 3 to 180 (178 residues), 90.3 bits, see alignment E=5.7e-29 PF13419: HAD_2" amino acids 5 to 186 (182 residues), 141.7 bits, see alignment E=7.1e-45 PF12710: HAD" amino acids 5 to 176 (172 residues), 37.1 bits, see alignment E=1.2e-12 TIGR01662: HAD hydrolase, family IIIA" amino acids 80 to 186 (107 residues), 46.8 bits, see alignment E=4.7e-16 TIGR01549: HAD hydrolase, family IA, variant 1" amino acids 90 to 180 (91 residues), 34.9 bits, see alignment E=2.8e-12 PF13242: Hydrolase_like" amino acids 143 to 210 (68 residues), 38.8 bits, see alignment E=1.8e-13

Best Hits

Swiss-Prot: 32% identical to GPH_HAEI8: Phosphoglycolate phosphatase (NTHI0697) from Haemophilus influenzae (strain 86-028NP)

KEGG orthology group: K01091, phosphoglycolate phosphatase [EC: 3.1.3.18] (inferred from 99% identity to bvu:BVU_1972)

Predicted SEED Role

"Phosphoglycolate phosphatase (EC 3.1.3.18)" in subsystem 2-phosphoglycolate salvage or Glycolate, glyoxylate interconversions or Photorespiration (oxidative C2 cycle) (EC 3.1.3.18)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 3.1.3.18

Use Curated BLAST to search for 3.1.3.18

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See I9UG07 at UniProt or InterPro

Protein Sequence (217 amino acids)

>HMPREF1058_RS14395 HAD family hydrolase (Phocaeicola vulgatus CL09T03C04)
MKKLVIFDLDGTLLNTIADLAAATNQALQYYGYPTHETEAYRFFVGNGINKLFERALPEG
ERTKENVLKIRSQFIPYYDEHNADLSRPYPGISELLKTLQQQGIMIAVASNKYQAATRKL
IAHYFPEINFVEVLGQREGIPAKPDPSIVNEIMTKAGVKQEDILYVGDSNVDMQTAHHAG
VTAIGVAWGFRPRTELEALHPAHIIEKAEELLPLLLS