Protein Info for HMPREF1058_RS13445 in Phocaeicola vulgatus CL09T03C04

Annotation: glycoside hydrolase family 2

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 850 900 955 signal peptide" amino acids 1 to 22 (22 residues), see Phobius details PF02837: Glyco_hydro_2_N" amino acids 27 to 185 (159 residues), 82.6 bits, see alignment E=8.4e-27 PF00703: Glyco_hydro_2" amino acids 190 to 307 (118 residues), 46.1 bits, see alignment E=1.9e-15 PF02836: Glyco_hydro_2_C" amino acids 313 to 540 (228 residues), 140.6 bits, see alignment E=1.5e-44 PF02929: Bgal_small_N" amino acids 710 to 871 (162 residues), 69.7 bits, see alignment E=8.1e-23

Best Hits

Predicted SEED Role

"Beta-galactosidase (EC 3.2.1.23)" in subsystem Galactosylceramide and Sulfatide metabolism or Lactose and Galactose Uptake and Utilization or Lactose utilization (EC 3.2.1.23)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 3.2.1.23

Use Curated BLAST to search for 3.2.1.23

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See I9UEV6 at UniProt or InterPro

Protein Sequence (955 amino acids)

>HMPREF1058_RS13445 glycoside hydrolase family 2 (Phocaeicola vulgatus CL09T03C04)
MNLRTIFISSFLSLSSLGMLAQEQINLSGTWQFAFAANQKEADRLERFYTPDFAKSKFKS
TPVPSNWVLLGYEEPVYRGFKDNQAGEGFYVREFTIPQDWKDKRILLHFGGVWSSAEVWL
NGNELGRHDCGYTSFAFDVTNKLKVDEPNKLAVRVRQITREYKFDVCDDWTWGGIYRDVT
LEAMPAKRWIDDVVVQTTFDHLFQDANLDIRVMISDKHKNTLPGNYPSPGEPYKLCFTLT
DKEGKEVAQRQMTVPAHVSTDREICLSLPVEAPHQWTAETPYLYSLKVELLEKEVVTHTR
MERVGFRQISTDGGVFRINGQAVKLRGVNRHDEHPDVGRATTRKQWLEDLTLMKAANINY
LRLSHYTPAEGFIELCDSMGMYVGNEVTLGGAGDLMYDPSFSEAVLQRSYETIVRDINKP
SIIYWSIGNEDPLTSLHMVSVKLVKALDPTRPVLLPWRPEEWLPKEVDILAPHYWNPQEY
DRLAGHSGRPVISTEYTHAYGNDAFGGLEARWKALTKHPAGAGAAVWMWADQGVKTPVRK
KEKDLSEDEYLRINTAGWDGIVDSYRNFTRDYWETKAVYAPVYPAVDRISFVPGQDSVRI
PIQNDFDFTNLSSVKMAWSVREDENVLYSGTDSMYGYPHTVSDFKLPVEKLVAVRPGKTY
YVWFVFTDEKGTEITRRAVELCPQTEQPVSVPVCRELLVTETDKVTIEAGDIRYVFSPKN
GQLVSAELKGKQLIKDLYPAIWRKLNQGETSGFGKENLRKAVDLTHYTSSVTAWKVEKTP
TNAVIRTTVDYRVDQENHFTVTYRYSIGVDGRLNVYYQILTKVAVPWLPIVGMSMQSVSG
LDQVHWLGLGPYDAYPNKQAAPILGVWGGTAGSSDVTGIKAMRWMERAGAEGTIHVSNSG
YMENDAMCPERTYILSGVFGRPEKGRRAEESVPQLRTDTGKPFVGEFSIMLKAVR