Protein Info for HMPREF1058_RS12720 in Phocaeicola vulgatus CL09T03C04

Annotation: primosomal protein N'

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 820 PF17764: PriA_3primeBD" amino acids 6 to 103 (98 residues), 99.7 bits, see alignment E=2e-32 PF04851: ResIII" amino acids 285 to 446 (162 residues), 52.2 bits, see alignment E=2e-17 PF00270: DEAD" amino acids 287 to 447 (161 residues), 64.3 bits, see alignment E=3.6e-21 TIGR00595: primosomal protein N'" amino acids 306 to 799 (494 residues), 576.1 bits, see alignment E=3e-177 PF18319: PriA_CRR" amino acids 536 to 560 (25 residues), 44.3 bits, see alignment (E = 4.2e-15) PF00271: Helicase_C" amino acids 602 to 679 (78 residues), 35.8 bits, see alignment E=2.5e-12 PF18074: PriA_C" amino acids 723 to 819 (97 residues), 29.7 bits, see alignment E=2.9e-10

Best Hits

KEGG orthology group: K04066, primosomal protein N' (replication factor Y) (superfamily II helicase) [EC: 3.6.4.-] (inferred from 100% identity to bvu:BVU_1471)

Predicted SEED Role

"Helicase PriA essential for oriC/DnaA-independent DNA replication" in subsystem Conserved gene cluster associated with Met-tRNA formyltransferase or DNA-replication

Isozymes

Compare fitness of predicted isozymes for: 3.6.4.-

Use Curated BLAST to search for 3.6.4.-

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See I8ZYD8 at UniProt or InterPro

Protein Sequence (820 amino acids)

>HMPREF1058_RS12720 primosomal protein N' (Phocaeicola vulgatus CL09T03C04)
MKKFVDVIVPLPIANQYTYSLPQEMEEMVQIGCRVVVPFGKKKFYTAIVTNVHYAAPEGY
ETKDIAEVLDSSPVLLPKQYEFWQWLAEYYLCTLGDVYKAAIPSGMKLESETLVEYNPDF
EATAPLPEKEQKILDLLSRDTEQCVTQLEKNSGLKNVLTVIKSLLDKEAIFVKEELKRNY
KPRTEARVRLVNGEADEAYLQRLFNELSRAPKQLMILMKYVELSGWVARGYALKEVTKKE
LLEKSGGSAAVFNGLVEKKVFEVYHQEIGRLDKGISDTGDINPLNIAQQQAYGNILQCFR
EKNVCLLHGVTSSGKTEIYIHLIQEVLKTGKQVLYLLPEIALTTQITERLKRVFGHRLGI
YHSKFPDAERVEIWQKQLGEKSYDVILGVRSSIFLPFRNLGLVIIDEEHENTYKQQDPAP
RYHARSSAIMLASMFKAKVLLGTATPGVETYFNATSGKYGLVELKERYKDIRLPHIELVD
IKELAHQKRMQGPFSPALVKQIKEALECKEQVILFQNRRGFAPMIECHTCGWVPKCKNCD
VSLTYHKGLNQLTCHYCGYTYQVPRSCPACGGVELMHRGFGTERIEDDIKLIFPEAKVAR
MDLDTTRTRTAYEKIIADFEQGKTDILIGTQMVSKGLDFDHVSVVGILNADTMLNFPDFR
SYERAFQLMAQVAGRAGRKNKQGLVILQTKSPDLPVIHQVMHNDYEQLYYDQLAERQMFK
YPPYYRLIYVYLKHRKEDILDLAADTMAAQLRSGLGDRVLGPDKPPVARIQTLFIKKMIV
KVEQNASIKKVRDYLLAVQRAILEDERFRSLLVYYDVDPQ