Protein Info for HMPREF1058_RS12315 in Phocaeicola vulgatus CL09T03C04

Annotation: glycoside hydrolase family 97 protein

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 738 signal peptide" amino acids 1 to 22 (22 residues), see Phobius details PF14508: GH97_N" amino acids 25 to 313 (289 residues), 259.6 bits, see alignment E=3.7e-81 PF10566: Glyco_hydro_97" amino acids 318 to 620 (303 residues), 365.3 bits, see alignment E=3.2e-113 PF14509: GH97_C" amino acids 623 to 724 (102 residues), 109.7 bits, see alignment E=1.1e-35

Best Hits

Swiss-Prot: 87% identical to SUSB_BACTN: Glucan 1,4-alpha-glucosidase SusB (susB) from Bacteroides thetaiotaomicron (strain ATCC 29148 / DSM 2079 / NCTC 10582 / E50 / VPI-5482)

KEGG orthology group: K01187, alpha-glucosidase [EC: 3.2.1.20] (inferred from 100% identity to bvu:BVU_1381)

Predicted SEED Role

"Alpha-glucosidase SusB (EC 3.2.1.20)" (EC 3.2.1.20)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 3.2.1.20

Use Curated BLAST to search for 3.2.1.20

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See I9UCV3 at UniProt or InterPro

Protein Sequence (738 amino acids)

>HMPREF1058_RS12315 glycoside hydrolase family 97 protein (Phocaeicola vulgatus CL09T03C04)
MNLKKNLSLIAFLCISFIANAQQKLNSPDGNLEMTFTLDGQGTPTYELTYKQKEVIKPSK
LGLELKKEDANAKTDFEWTDKKDIDKLDIKTNLYNGFEIKDVRTSTNDETWQPVWGEEKE
IRNHYNELAVTLNQPANDRFIIIRFRLFNDGLGFRYEFPQQKNLNYFVIKEEHSQFAMAG
DHTAYWIPGDYDTQEYDYTVSRLSEIRGLFSKAYTENSSQTAFSPTGVQTALMMKTDDGL
YINLHEAALIDYACMHLNLDDKNMIFESWLTPDAQGDKGYMQTPCTSPWRTVIVSDDARN
ILASRITLNLNEPCKIADTSWIKPVKYIGVWWDMITGKGSWAYTDDLSSVKLGETDYNKT
KPNGKHSANTANVKRYIDFAAEHGFDQVLVEGWNEGWEDWFGKSKDYVFDFVTPYPDFNV
KELQSYAASKGVKLMMHHETSASVRNYERHMDQAYQFMVDNGYNSVKSGYVGNIIPRGEH
HYGQWMDNHYLYAVKKAADYKIMVNAHEAVRPTGICRTYPNLIGNESARGTEYESFGGNN
VNHTTILPFTRLIGGPMDYTPGIFETRCNKMNPTNNSQVRSTLARQLALYVTMYSPLQMA
ADIPENYERFMDAFQFIKDVAIDWDETKYLEAEPGDYITIARKAKGTNDWYIGCTADENG
HSSKLIFDFLDPDKKYIATVYADAKDADWKDNPQAYTIRKGIVTNKSKLNLHAASGGGYA
ISIKEVKDKSELKGIKRL