Protein Info for HMPREF1058_RS12235 in Phocaeicola vulgatus CL09T03C04

Annotation: DUF4981 domain-containing protein

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 100 200 300 400 500 600 700 800 900 1000 1058 signal peptide" amino acids 1 to 21 (21 residues), see Phobius details PF02837: Glyco_hydro_2_N" amino acids 74 to 241 (168 residues), 152.9 bits, see alignment E=2.1e-48 PF00703: Glyco_hydro_2" amino acids 244 to 348 (105 residues), 39 bits, see alignment E=3e-13 PF02836: Glyco_hydro_2_C" amino acids 350 to 635 (286 residues), 325.6 bits, see alignment E=7.4e-101 PF16353: LacZ_4" amino acids 645 to 733 (89 residues), 87.6 bits, see alignment E=1.4e-28 PF02929: Bgal_small_N" amino acids 766 to 1040 (275 residues), 254.8 bits, see alignment E=2.5e-79

Best Hits

KEGG orthology group: K01190, beta-galactosidase [EC: 3.2.1.23] (inferred from 99% identity to bvu:BVU_1365)

Predicted SEED Role

"Beta-galactosidase (EC 3.2.1.23)" in subsystem Galactosylceramide and Sulfatide metabolism or Lactose and Galactose Uptake and Utilization or Lactose utilization (EC 3.2.1.23)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 3.2.1.23

Use Curated BLAST to search for 3.2.1.23

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See I8ZWR0 at UniProt or InterPro

Protein Sequence (1058 amino acids)

>HMPREF1058_RS12235 DUF4981 domain-containing protein (Phocaeicola vulgatus CL09T03C04)
MKHSLHTVLTLLFVCASAWGENVPWQNPQINEINREPAHAHFIPYTNEANALKQQALPAA
QRFAVNPATERRISLDGTWKFLFSKNNEECPTDFYKMGYNTKRWKDIQVPGSWELQGFDS
PIYTDVAYPFPANPPHVPTDYNPVGAYVREFTVPAHWKGMDIFLDFEGVESAFYCWVNGE
LAGYSEDSRLPAHFNITPFLKAGKNKLAVKVFRYSDGSYLEDQDYWKYSGIERDVYLYAR
PQSRVQDFKLVAGLTNGYKDGDFNLDITLHKPHPGGTVEVKVMDKGNVIYQHKKEITSAT
DTLFAQKHLFPAILPWNAETPNLYTLVVNTYNAQGKALESFTQPFGFRSVEMRNGMQLIN
GVAVLFKGVNRHEHDPHHGRTITVESMIKDIQLMKQFNLNAVRTCHYPNRYEWYALCNEY
GLYLVDEANIESHGMQAHKDGTLANNPDWEVPFMQRMSRMVLRDRNITAIVTWSMGNESG
YGKHFETLYDWTKKFDPTRPVQYEGGGYDAKSDIYCPMYARVWALRRHVNQRDARPMILC
EYAHAMGNSVGNLQDYWDLIYKYDQLQGGFIWDWVDATFDIKDKNGNKIWAYGGDMGFVG
VPNDSNFCANGLVAADRSLHPHIWEVKKVYQYIHFDPVAFTTKQIKVTNWHDFIGLEDYK
LHWTVETDGKTVQSGEMDFPVMAARSSAFITIPMNLIPNDGKEYFLKLEAFTKKEAPLVP
KGHQVAMEQWQLNPEGERLLNATWTARELVDKTVNTERTPDAITLTGENFRIAFSTADGE
MTELLYNGKNLIKEGLQPNFWRALTDNDVANNHLERCGIWKFAGKNAKLQSIDLKEDSNK
QLATVTVNYKLEAQESTLQTVYQVRPNGAVKVSMHFVPGNKSLPEMPRLGMRMILPAEYD
VMTWLGRGPQENYADRKTGYPIGLYTATIWEQFHPYVRAQETGNKTEVRWVALRNKVGEG
LLITGEEPLNVSAWNFPLEDIDYVAFNTERRHGGSIMKKDMIWLNIDHRHMGVGGDNTWG
AQVHPEYTITPHEWQYSFTMQPLSNQDDAAEKAHKKWF