Protein Info for HMPREF1058_RS11250 in Phocaeicola vulgatus CL09T03C04

Annotation: leucine--tRNA ligase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 850 900 944 PF00133: tRNA-synt_1" amino acids 11 to 144 (134 residues), 50.3 bits, see alignment E=3.4e-17 PF09334: tRNA-synt_1g" amino acids 35 to 164 (130 residues), 50.3 bits, see alignment E=3.4e-17 TIGR00396: leucine--tRNA ligase" amino acids 201 to 944 (744 residues), 700.1 bits, see alignment E=2.1e-214 PF13603: tRNA-synt_1_2" amino acids 276 to 440 (165 residues), 192.4 bits, see alignment E=1.1e-60 PF08264: Anticodon_1" amino acids 795 to 906 (112 residues), 71 bits, see alignment E=2.1e-23

Best Hits

Swiss-Prot: 100% identical to SYL_BACV8: Leucine--tRNA ligase (leuS) from Bacteroides vulgatus (strain ATCC 8482 / DSM 1447 / JCM 5826 / NBRC 14291 / NCTC 11154)

KEGG orthology group: K01869, leucyl-tRNA synthetase [EC: 6.1.1.4] (inferred from 100% identity to bvu:BVU_1149)

Predicted SEED Role

"Leucyl-tRNA synthetase (EC 6.1.1.4)" (EC 6.1.1.4)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 6.1.1.4

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See I9UC35 at UniProt or InterPro

Protein Sequence (944 amino acids)

>HMPREF1058_RS11250 leucine--tRNA ligase (Phocaeicola vulgatus CL09T03C04)
MEYNFREIEKKWQQRWAENHTYQVTEDESKKKFYVLNMFPYPSGAGLHVGHPLGYIASDI
YARYKRLQGFNVLNPMGYDAYGLPAEQYAIQTGQHPAITTVNNINRYREQLDKIGFSFDW
NREVRTCEPGYYHWTQWAFQQMFNSYYCNDTQQARPISELTEAFARYGNEGLNAACSEEL
SFTAEEWNAKSEKEQQEILMNYRIAYLGETMVNWCPQLGTVLANDEVVDGVSERGGFPVV
QKKMRQWCLRVSAYAQRLLDGLDTVDWTDSLKETQRNWIGRSEGTEVQFKVKDSDIEFTI
FTTRADTMFGVTFMVLAPESELVPQLTTEAQKAEVEAYLDRTKKRTERERIADRRVTGVF
SGSYAINPFTGEAVPVWISDYVLAGYGTGAIMAVPAHDSRDYAFAKHFNLPIVPLVEGCD
VSEESFDAKEGIVCNSPRKDVTPYCDLSLNGLTIKEAIAATKEYVKAHNLGRVKVNYRLR
DAIFSRQRYWGEPFPVYYKNGMPYMIDSSKLPLELPEVAKFLPTETGEPPLGHATKWAWD
VEKGEVVENNLIDNVTIFPLELNTMPGFAGSSAYYLRYMDPHNAQALVSEKADHYWQNVD
LYVGGTEHATGHLIYSRFWNKFLHDLGVSIKEEPFQKLVNQGMIQGRSNFVYRIKDTNTF
VSLNLKDQYETTPLHVDVNIVSNDILDVEAFKAWRPEYNDAEFILEDGKYICGWAVEKMS
KSMYNVVNPDMIVEKYGADTLRMYEMFLGPVEQSKPWDTNGIDGVHRFLKKLWNLFYSRT
DEFLPVEGEPTKEELKAIHKLIKKVTGDIETFSYNTSISAFMICVNELTSLKCRNKEVLS
NLIILLAPFAPHYAEELWEALGNTTSVCDAQWPAFNEDYLKEDTVKYTISFNGKARFTMD
FAADADNNTIQTTVMADEQAQKWIEGKTPKKVIIVPKKIVNIVL