Protein Info for HMPREF1058_RS09160 in Phocaeicola vulgatus CL09T03C04

Annotation: O-acetylhomoserine aminocarboxypropyltransferase/cysteine synthase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

Best Hits

Swiss-Prot: 46% identical to CYSD_EMENI: Homocysteine synthase (cysD) from Emericella nidulans (strain FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139)

KEGG orthology group: K01740, O-acetylhomoserine (thiol)-lyase [EC: 2.5.1.49] (inferred from 100% identity to bvu:BVU_0770)

MetaCyc: 45% identical to O-acetylhomoserine sulfhydrylase (Leptospira meyeri)
O-acetylhomoserine aminocarboxypropyltransferase. [EC: 2.5.1.49]

Predicted SEED Role

"O-acetylhomoserine sulfhydrylase (EC 2.5.1.49) / O-succinylhomoserine sulfhydrylase (EC 2.5.1.48)" in subsystem Methionine Biosynthesis (EC 2.5.1.48, EC 2.5.1.49)

MetaCyc Pathways

superpathway of L-lysine, L-threonine and L-methionine biosynthesis II (12/15 steps found)
aspartate superpathway (19/25 steps found)
superpathway of S-adenosyl-L-methionine biosynthesis (7/9 steps found)
superpathway of L-methionine biosynthesis (transsulfuration) (7/9 steps found)
superpathway of L-homoserine and L-methionine biosynthesis (6/8 steps found)
L-homocysteine biosynthesis (1/2 steps found)
L-methionine biosynthesis I (3/5 steps found)
superpathway of L-methionine biosynthesis (by sulfhydrylation) (8/12 steps found)
L-methionine biosynthesis III (2/4 steps found)
superpathway of L-lysine, L-threonine and L-methionine biosynthesis I (12/18 steps found)
L-methionine biosynthesis II (3/6 steps found)
homocysteine and cysteine interconversion (1/4 steps found)
superpathway of L-cysteine biosynthesis (fungi) (2/6 steps found)
S-methyl-5-thio-α-D-ribose 1-phosphate degradation II (1/5 steps found)
S-methyl-5-thio-α-D-ribose 1-phosphate degradation III (1/5 steps found)
superpathway of sulfur amino acid biosynthesis (Saccharomyces cerevisiae) (4/10 steps found)

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 2.5.1.48, 2.5.1.49

Use Curated BLAST to search for 2.5.1.48 or 2.5.1.49

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See I9U9H7 at UniProt or InterPro

Protein Sequence (425 amino acids)

>HMPREF1058_RS09160 O-acetylhomoserine aminocarboxypropyltransferase/cysteine synthase (Phocaeicola vulgatus CL09T03C04)
MDKKYSRETLCVQAGWTPKKGEPRVLPIYQSTTFKYDTSEQMARLFDLEDSGYFYTRLQN
PTNDAVAAKIAALEGGVGAMLTSSGQAANFYAIFNICQAGDHFVCSSTIYGGTFNLFGVT
LKKLGIECTFIDANASEEEIDKAFRPNTKALFGETISNPSIDVLDIEKFARIAHKHGVPL
IVDNTFATPINCRPFEWGADIVTHSTTKYMDGHATSVGGAIVDSGNFDWEAHADKFPGLT
QPDESYHGLTYTKAFGKMAYMTKATAQLMRDLGSIQSPQNAFLLNLGLETLHLRVPRHCE
NAQKVAEWLEANPKVKWVNYCGLKSSKYYDLAQKYMPNGSCGVIAFGLNGTREEAIEFMD
RLKFICIVTHVADARTCVLHPASHTHRQLTDEQLREAGVAPDLIRLSVGIENVSDIIADI
EQALQ