Protein Info for HMPREF1058_RS08470 in Phocaeicola vulgatus CL09T03C04

Annotation: UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D- alanine ligase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF01225: Mur_ligase
  • amino acids 16 to 85 (70 residues), 26.7 bits, E=8.7e-10
• TIGR01143: UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-alanine ligase
  • amino acids 18 to 432 (415 residues), 399 bits, E=1.3e-123
• PF08245: Mur_ligase_M
  • amino acids 97 to 283 (187 residues), 149.9 bits, E=1.4e-47
• PF02875: Mur_ligase_C
  • amino acids 307 to 371 (65 residues), 41 bits, E=2.9e-14

Best Hits

KEGG orthology group: K01929, UDP-N-acetylmuramoylalanyl-D-glutamyl-2,6-diaminopimelate--D-alanyl-D-alanine ligase [EC: 6.3.2.10] (inferred from 99% identity to bvu:BVU_0516)

Predicted SEED Role

"UDP-N-acetylmuramoylalanyl-D-glutamyl-2,6-diaminopimelate--D-alanyl-D-alanine ligase (EC 6.3.2.10)" in subsystem Methicillin resistance in Staphylococci or Peptidoglycan Biosynthesis (EC 6.3.2.10)

MetaCyc Pathways

• peptidoglycan biosynthesis I (meso-diaminopimelate containing) (11/12 steps found)
• UDP-N-acetylmuramoyl-pentapeptide biosynthesis I (meso-diaminopimelate containing) (8/8 steps found)
• UDP-N-acetylmuramoyl-pentapeptide biosynthesis II (lysine-containing) (7/8 steps found)
• UDP-N-acetylmuramoyl-pentapeptide biosynthesis III (meso-diaminopimelate containing) (6/8 steps found)
• peptidoglycan biosynthesis III (mycobacteria) (11/15 steps found)
• peptidoglycan biosynthesis II (staphylococci) (12/17 steps found)
• peptidoglycan biosynthesis IV (Enterococcus faecium) (12/17 steps found)
• peptidoglycan biosynthesis V (β-lactam resistance) (11/17 steps found)

KEGG Metabolic Maps

• Lysine biosynthesis
• Peptidoglycan biosynthesis

Isozymes

Compare fitness of predicted isozymes for: 6.3.2.10

Use Curated BLAST to search for 6.3.2.10

Sequence Analysis Tools

See I9U6U8 at UniProt or InterPro

Protein Sequence (432 amino acids)

>HMPREF1058_RS08470 UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D- alanine ligase (Phocaeicola vulgatus CL09T03C04)
MDISALYKCFTECGKVTTDSRNCPEGSMFIALKGETFNGNAYALQALEKGCQYAVVDEKE
YAADGHPRILLVDDCLKALQELANYHRHKMGTRIIGITGTNGKTTTKELIASVLQKRYEV
LYTQGNLNNHIGVPLTLLRLTPEHELAVIEMGANHPGEIKALTRIVEPDFGMITNVGKAH
LEGFGSFEGVIKTKGELYEHLRYNGKHIIFIDKDNEFLTAISQGLTRICYGMEGGEGLYI
SGKLGACAPFLSFEWEHEGQKFEVNTHLIGSYNMKNALAAITVGRFFSVPSRQICEALEA
YVPHNNRSQLTETTDNKLIVDAYNANPTSMMAALENFRLMNVAHKMVILGDMKELGETSH
EEHQKIADYLDECKFDRVLLAGEEFGKVIPSFEHFKDVVALKESLERYKPKGYYILIKGS
NSMKLSQLQEIL