Protein Info for HMPREF1058_RS07940 in Phocaeicola vulgatus CL09T03C04

Annotation: dTDP-4-dehydrorhamnose reductase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 285 PF04321: RmlD_sub_bind" amino acids 3 to 283 (281 residues), 328.1 bits, see alignment E=8.4e-102 TIGR01214: dTDP-4-dehydrorhamnose reductase" amino acids 4 to 282 (279 residues), 297.5 bits, see alignment E=4.2e-93 PF01370: Epimerase" amino acids 4 to 208 (205 residues), 71 bits, see alignment E=2.1e-23 PF16363: GDP_Man_Dehyd" amino acids 34 to 151 (118 residues), 50.3 bits, see alignment E=5.3e-17 PF02719: Polysacc_synt_2" amino acids 36 to 159 (124 residues), 27.5 bits, see alignment E=3.6e-10

Best Hits

Swiss-Prot: 41% identical to RMLD_ECOLI: dTDP-4-dehydrorhamnose reductase (rfbD) from Escherichia coli (strain K12)

KEGG orthology group: K00067, dTDP-4-dehydrorhamnose reductase [EC: 1.1.1.133] (inferred from 100% identity to bvu:BVU_0075)

Predicted SEED Role

"dTDP-4-dehydrorhamnose reductase (EC 1.1.1.133)" in subsystem Rhamnose containing glycans or dTDP-rhamnose synthesis or linker unit-arabinogalactan synthesis (EC 1.1.1.133)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 1.1.1.133

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See I8ZQ50 at UniProt or InterPro

Protein Sequence (285 amino acids)

>HMPREF1058_RS07940 dTDP-4-dehydrorhamnose reductase (Phocaeicola vulgatus CL09T03C04)
MKNILVTGANGQLGNEMRVLSAEYKEYTCFFTDVAELDICDEQAVMTFVKENNIHVIVNC
AAYTAVDKAEDDIELCTKLNKNAVSYLAKAAEANWGEFIQISTDYVFDGTKHLPYNEGDV
PCPNSVYGKTKLAGETNALEYCKKTMIIRTAWLYSTFGNNFVKTMLRLGKEKETLGVVFD
QIGTPTYARDLARAIFTAIYKGVVPGVYHFSDEGVCSWYDFTKAIHRIAGITTCKVSPLH
TNEYPAKAPRPHYSVLDKTKIKTTYNIEIPHWEESLEACIKELNA