Protein Info for HMPREF1058_RS05180 in Phocaeicola vulgatus CL09T03C04

Annotation: RluA family pseudouridine synthase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 540 PF00849: PseudoU_synth_2" amino acids 345 to 493 (149 residues), 117.5 bits, see alignment E=3.2e-38

Best Hits

KEGG orthology group: K06177, ribosomal large subunit pseudouridine synthase A [EC: 5.4.99.12] (inferred from 100% identity to bvu:BVU_3779)

Predicted SEED Role

"Ribosomal large subunit pseudouridine synthase A (EC 4.2.1.70)" in subsystem Ribosome biogenesis bacterial (EC 4.2.1.70)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 4.2.1.70, 5.4.99.12

Use Curated BLAST to search for 4.2.1.70 or 5.4.99.12

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See I9U2C1 at UniProt or InterPro

Protein Sequence (540 amino acids)

>HMPREF1058_RS05180 RluA family pseudouridine synthase (Phocaeicola vulgatus CL09T03C04)
MIHFFKNSIATIKLPDKFTYPFHYTPHPLCIIATKEVQAYLTSQSQWQKELQQGKMFGVL
IVQTPENKIGYLAAFSGTLAGKNCHPFFVPPIYDLLQPQGFFKIEEKRISAINVCIKKTQ
NDPRYIDLLRQIEKEKIQSQQELTEAKEFFKSAKKNREIRRKTGIPDAKELAAMIRESQF
QKAELKRMEKIWKEKIASLQAEADTFITKIETMKIERKKRSATLQRKLFEQFQILNAHGE
TKDLCRIFAQTIQKFPPAGAGECAAPKLLQYAYKHQLKPIAMAEFWWGDSPKAEIRHHGY
YYPACKGKCGPILGHMLQGLEVEENPLLKKHYHEMPLEIVYEDNYLVVINKPAGMLSVPG
KGEIDSVYQHIKILYPDATGPLIVHRLDMATSGVLLIAKNKEVHQHLQAQFKNRMIKKRY
IALLDGKISSKEGTIILPLRMDPLDRPRQVVDHEHGKTAITQYQVLNEQEGNTLIAFYPL
TGRTHQLRVHAAHPEGLHCPIRGDELYGQKADRLYLHAESLEFVHPVTKEIIFVEKKSNF