Protein Info for HMPREF1058_RS04280 in Phocaeicola vulgatus CL09T03C04

Annotation: family 20 glycosylhydrolase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 773 signal peptide" amino acids 1 to 23 (23 residues), see Phobius details PF02838: Glyco_hydro_20b" amino acids 32 to 162 (131 residues), 101.1 bits, see alignment E=1.9e-32 PF00728: Glyco_hydro_20" amino acids 165 to 508 (344 residues), 366.9 bits, see alignment E=3.1e-113 PF13287: Fn3_assoc" amino acids 557 to 613 (57 residues), 59.5 bits, see alignment 6.9e-20 PF13290: CHB_HEX_C_1" amino acids 557 to 606 (50 residues), 45.7 bits, see alignment 1.3e-15 PF00754: F5_F8_type_C" amino acids 639 to 746 (108 residues), 34.8 bits, see alignment E=4.1e-12

Best Hits

KEGG orthology group: K12373, hexosaminidase [EC: 3.2.1.52] (inferred from 100% identity to bvu:BVU_3245)

Predicted SEED Role

"Beta-hexosaminidase (EC 3.2.1.52)" in subsystem Chitin and N-acetylglucosamine utilization or N-Acetyl-Galactosamine and Galactosamine Utilization (EC 3.2.1.52)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 3.2.1.52

Use Curated BLAST to search for 3.2.1.52

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See I8ZIG7 at UniProt or InterPro

Protein Sequence (773 amino acids)

>HMPREF1058_RS04280 family 20 glycosylhydrolase (Phocaeicola vulgatus CL09T03C04)
MKKQLMQWAGVGMWMVLGLSACSPTKTEIGNLNVIPQPQEVSQDIQAHPFVINPQTGIVY
PEGNEKLQRTAEFLASYIEEATGITVRTTTEAARKNSIILAVDGSITNKEGYQLEVTSEN
IHLNGGSESGVFYGIQTLYKALPLTKNKQVSAAIPVGTVNDYPRFGYRGFMVDVGRHYFP
VSYLKQIIDMLALHNINYFHWHLTEDQGWRIEIKKYPKLTEIGSMRPRTLIDRETQTYDE
TPHSGFYTQEEAKEIVKYAADRFITVIPEVDLPGHMMGALVSYPELGCTGGPYEIPCKWG
VFPDVLCGGNDRTLQFAKDVLNEIMDIFPSPYIHIGGDECPKVRWEKCPVCQAKIRELGL
KDTPKHSKENQLQTYFMSEVGKVINDRGRKMLGWDEMLEGGLAPGATVMSWTGVKGGIEA
ARLHHDAIMTPIQYLYFSNPTYNRIKGTKSLGRVYTFEPVSNELAEDERKYIIGTQGCIW
TEWTRDSLKMEWQILPRMAALSEIQWTEPSHKNFDSFLKRLPALLAIYRNRGYDFRQDIY
DVNIDIVPAPDEGKARIAFQTFDDAEIHYTLDGSVPDVQSPLYTDTIQVDKDVIIQAIAV
RPQGASQISKEEIHFNAATMKPVTLNTIPHKSYTFKGGSTLIDGLYGDMNYRSGRWIGFY
GTDMNVTLDLLEPKEVSSVFVNTMLNTGDAIFGTTGLKVEVSEDGKNFRRVASEDFPVVE
KGTKMQSRKDSVSFDKVKARYIKIIAEVTPKLPAWHSMPGEKAFLFVDEIGVE