Protein Info for HMPREF1058_RS03920 in Phocaeicola vulgatus CL09T03C04
Annotation: proline--tRNA ligase
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
Swiss-Prot: 100% identical to SYP_BACV8: Proline--tRNA ligase (proS) from Bacteroides vulgatus (strain ATCC 8482 / DSM 1447 / JCM 5826 / NBRC 14291 / NCTC 11154)
KEGG orthology group: K01881, prolyl-tRNA synthetase [EC: 6.1.1.15] (inferred from 100% identity to bvu:BVU_3320)Predicted SEED Role
"Prolyl-tRNA synthetase (EC 6.1.1.15), archaeal/eukaryal type" (EC 6.1.1.15)
MetaCyc Pathways
- tRNA charging (21/21 steps found)
- pyoluteorin biosynthesis (1/7 steps found)
- ergotamine biosynthesis (4/12 steps found)
- (2S,3E)-2-amino-4-methoxy-but-3-enoate biosynthesis (2/10 steps found)
- clorobiocin biosynthesis (1/10 steps found)
- superpathway of ergotamine biosynthesis (5/18 steps found)
- pyrrolomycin biosynthesis (1/14 steps found)
- sulfazecin biosynthesis (1/16 steps found)
- phosalacine biosynthesis (2/25 steps found)
- phosphinothricin tripeptide biosynthesis (2/25 steps found)
- colibactin biosynthesis (7/38 steps found)
KEGG Metabolic Maps
Isozymes
No predicted isozymesUse Curated BLAST to search for 6.1.1.15
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
See I8ZIK6 at UniProt or InterPro
Protein Sequence (496 amino acids)
>HMPREF1058_RS03920 proline--tRNA ligase (Phocaeicola vulgatus CL09T03C04) MAVELKELTKRSENYSQWYNDLVVKADLAEQSPVRGCMVIKPYGYAIWEKMQRQLDDMFK ETGHVNAYFPLLIPKSYLSREAEHVEGFAKECAVVTHYRLKNAEDGSGVIVDPAAKLEEE LIIRPTSETIIWSTYKNWINSYRDLPILCNQWANVMRWEMRTRLFLRTAEFLWQEGHTAH ATREEAEAEAQKMLHVYGDFAEKYMAVPVIKGVKSANERFAGALDTYTIEGLMQDGKALQ CGTSHFLGQNFAKAFNVQFVDKNNKLDYVWATSWGVSTRLMGALIMTHSDDNGLVLPPHL APIQVVIVPIYKNDEMLKKIDAKVEGIVNKLKAMGISVKYDNADNKRPGFKFADYELKGV PVRLVMGGRDLENNTMEVMRRDTLEKETRSCDGIEEYVQQLLEDIQNNIYQKALNYRNEH IVKVDSYDDFKEQIEKGGFILAHWDGTPETEDRIKEETKATIRCLPFDADEESLTPGKCM VTGKPSARRVLFARAY