Protein Info for HMPREF1058_RS03520 in Phocaeicola vulgatus CL09T03C04

Annotation: replicative DNA helicase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 518 PF00772: DnaB" amino acids 26 to 126 (101 residues), 109.5 bits, see alignment E=1.2e-35 TIGR00665: replicative DNA helicase" amino acids 26 to 465 (440 residues), 538.3 bits, see alignment E=6.6e-166 PF13481: AAA_25" amino acids 201 to 378 (178 residues), 28 bits, see alignment E=2.5e-10 PF03796: DnaB_C" amino acids 201 to 464 (264 residues), 358.7 bits, see alignment E=2.5e-111

Best Hits

Swiss-Prot: 44% identical to DNAC_BACSU: Replicative DNA helicase (dnaC) from Bacillus subtilis (strain 168)

KEGG orthology group: K02314, replicative DNA helicase [EC: 3.6.4.12] (inferred from 100% identity to bvu:BVU_3467)

Predicted SEED Role

"Replicative DNA helicase (EC 3.6.1.-)" in subsystem DNA-replication (EC 3.6.1.-)

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 3.6.1.-, 3.6.4.12

Use Curated BLAST to search for 3.6.1.- or 3.6.4.12

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See I9TYM3 at UniProt or InterPro

Protein Sequence (518 amino acids)

>HMPREF1058_RS03520 replicative DNA helicase (Phocaeicola vulgatus CL09T03C04)
MAETRKMTRTPKTSKAKPVDEYGHLQPQAPELEEAVLGALMIEKDAYSLVSEILRPESFY
ERRHQLIYSAITSLALRQQPVDILTVAEQLRSTGELEDAGGPFYITQLSGKVASSAHIEY
HARIIAQKFLARELITFTSNIQTKAFDETQDVDDLMQEAEGKLFEISQQNMKKDYTQINP
VIQEAYEMLQKAAARTDGLSGLESGFHALDKMTSGWQNSDLVIIAARPAMGKTAFVLSMA
KNMAVNAKIPVALFSLEMSNVQLVNRMIVNVCEIPGEKIKSGQLAPYEWGQLDYKIKELY
DAPMYVDDTPSLSVFELRTKARRLVREHGVKIIIIDYLQLMNASGMSFGSRQEEVSTISR
SLKGLAKELNIPIIALSQLNRGVESREGIDGKRPQLSDLRESGAIEQDADMVCFIHRPEY
YKIYSDEKGNDLHGMAEIIIAKHRNGAVGDVLLRFRGEFARFQNPDDDVIVPMPGEAPGI
IRSKMNGGGNSVPPPSPDAAPADNNPFGAPIPEGPLPF