Protein Info for HMPREF1058_RS03515 in Phocaeicola vulgatus CL09T03C04

Annotation: phenylalanine--tRNA ligase subunit beta

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 820 TIGR00472: phenylalanine--tRNA ligase, beta subunit" amino acids 1 to 820 (820 residues), 877.5 bits, see alignment E=4.7e-268 PF01588: tRNA_bind" amino acids 48 to 151 (104 residues), 77 bits, see alignment E=2.5e-25 PF03483: B3_4" amino acids 225 to 398 (174 residues), 227.6 bits, see alignment E=2e-71 PF03484: B5" amino acids 418 to 484 (67 residues), 73.8 bits, see alignment E=2.7e-24 PF17759: tRNA_synthFbeta" amino acids 489 to 709 (221 residues), 160.4 bits, see alignment E=1.1e-50 PF03147: FDX-ACB" amino acids 727 to 820 (94 residues), 109.5 bits, see alignment E=2.3e-35

Best Hits

Swiss-Prot: 83% identical to SYFB_BACTN: Phenylalanine--tRNA ligase beta subunit (pheT) from Bacteroides thetaiotaomicron (strain ATCC 29148 / DSM 2079 / NCTC 10582 / E50 / VPI-5482)

KEGG orthology group: K01890, phenylalanyl-tRNA synthetase beta chain [EC: 6.1.1.20] (inferred from 100% identity to bvu:BVU_3468)

Predicted SEED Role

"Phenylalanyl-tRNA synthetase beta chain (EC 6.1.1.20)" (EC 6.1.1.20)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 6.1.1.20

Use Curated BLAST to search for 6.1.1.20

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See I8ZIS1 at UniProt or InterPro

Protein Sequence (820 amino acids)

>HMPREF1058_RS03515 phenylalanine--tRNA ligase subunit beta (Phocaeicola vulgatus CL09T03C04)
MNISYNWLKEYVNFDLTPDEVAEALTSIGLETGGVEEVQTIKGGLEGIVIGEVLTCEPHP
NSDHMHVTTVNLGQGEPVQIVCGAANVAAGQKVVVATLGTKLYDGEECFTIKKSKLRGIE
SMGMICAEDEIGIGNSHEGIIVLPADAVPGTPAKDYFNIKSEYVLEVDITPNRADACSHY
GVARDLYAYLIQNGKQATLKRPSVEAFKVDNHDMDIAIEVENTEACPRYAGVSIKGVTVK
ESPEWLQNKLRLIGVRPINNIVDITNYILHAYGQPLHCFDADKIKGGKIVVKTVAEGTTF
VTLDEVERKLSDKDLMICNTEEPMCIAGVFGGLDSGTTEQTVDVFLESAYFNPTWVRKTA
RRHGLSTDSSFRFERGIDPNGTIYALKEAALLVQELAGGTISSEIKDNYPAPIADFTVEL
NYEKVHSLIGKTIPVETIKSIVTSLEMKIVNETPEGLTLQVPPYRVDVQRDCDVVEDILR
IYGYNNVEIPTTLKSSLTTKGEVDYSQKLQNLISEQLVGCGFNEILNNSLTAASYYEGSE
TYKNENLVYLMNPLSNDLNVMRQTLLFGGLESIQHNANRKNADLKFFEFGNCYFYNAEKK
NPEKVLAAYAEEYHLGLWVTGKRVSNSWAHPDENSSVYELKAYVLNIFTRLGLNFGNVVF
GNLTNDIYSTAISVHTRGGKLLATFGIISKKIQKAFDIDNEVYYAEINWKELMKAIKSAK
VNFKELSKFPAVKRDLALLIDKNIQFAEIEKIAYETEKKLLKEVELFDVYEGKNLEPGKK
SYAVSFLLQDESATLNDKQIDKIMSKLIANLENKLGAKLR