Protein Info for HMPREF1058_RS02880 in Phocaeicola vulgatus CL09T03C04

Annotation: tRNA 2-thiouridine(34) synthase MnmA

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 354 TIGR00420: tRNA (5-methylaminomethyl-2-thiouridylate)-methyltransferase" amino acids 1 to 351 (351 residues), 341.9 bits, see alignment E=1.8e-106 PF03054: tRNA_Me_trans" amino acids 1 to 196 (196 residues), 191.8 bits, see alignment E=1.7e-60 PF20259: tRNA_Me_trans_M" amino acids 201 to 263 (63 residues), 88.1 bits, see alignment E=3.3e-29 PF20258: tRNA_Me_trans_C" amino acids 275 to 351 (77 residues), 41.3 bits, see alignment E=2.5e-14

Best Hits

Swiss-Prot: 100% identical to MNMA1_BACV8: tRNA-specific 2-thiouridylase MnmA 1 (mnmA1) from Bacteroides vulgatus (strain ATCC 8482 / DSM 1447 / JCM 5826 / NBRC 14291 / NCTC 11154)

KEGG orthology group: K00566, tRNA-specific 2-thiouridylase [EC: 2.8.1.-] (inferred from 100% identity to bvu:BVU_0403)

Predicted SEED Role

"tRNA-specific 2-thiouridylase MnmA"

Isozymes

Compare fitness of predicted isozymes for: 2.8.1.-

Use Curated BLAST to search for 2.8.1.-

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See I9ILX8 at UniProt or InterPro

Protein Sequence (354 amino acids)

>HMPREF1058_RS02880 tRNA 2-thiouridine(34) synthase MnmA (Phocaeicola vulgatus CL09T03C04)
MNIAALISGGVDSAVVVHLLCEQGYKPDLFYIKIGMDGDEDLTCTAEEDIELCTATARKY
GLSLNIIDLHRQYWDNVVAYAIDKVKRGLTPNPDVMCNKLIKFGCFEQEAGHLYDKTATG
HYASILEKDGKIWLGTAQDPVKDQTDFLAQIDYLQISKLMFPLGGLMKNEVRDIARIAKL
PSAQRKDSQGICFLGKINYNEFLCRFLGEKEGDIIEMETGKRIGTHKGYWFHTIGQRKGL
GLGGGPWFVIRKDIDENIIYVSHGYDTDKQYGTDFALHDFHFITEDLWKGAPSADVSFKI
RHTDTFMKGILTREDNLFRIHSHVPLQGIAPGQFGVLYDKNAEICVGSGEITLS