Protein Info for HMPREF1058_RS02700 in Phocaeicola vulgatus CL09T03C04

Annotation: glycerol kinase GlpK

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 498 PF00370: FGGY_N" amino acids 5 to 251 (247 residues), 216.2 bits, see alignment E=5.5e-68 PF02782: FGGY_C" amino acids 262 to 449 (188 residues), 126.6 bits, see alignment E=1.1e-40

Best Hits

Swiss-Prot: 48% identical to GLPK2_THEMA: Glycerol kinase 2 (glpK2) from Thermotoga maritima (strain ATCC 43589 / MSB8 / DSM 3109 / JCM 10099)

KEGG orthology group: K00864, glycerol kinase [EC: 2.7.1.30] (inferred from 100% identity to bvu:BVU_0357)

MetaCyc: 49% identical to glycerol kinase (Escherichia coli K-12 substr. MG1655)
Glycerol kinase. [EC: 2.7.1.30]

Predicted SEED Role

"Glycerol kinase (EC 2.7.1.30)" in subsystem Glycerol and Glycerol-3-phosphate Uptake and Utilization or Glycerolipid and Glycerophospholipid Metabolism in Bacteria or MLST (EC 2.7.1.30)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.7.1.30

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See I9TXJ5 at UniProt or InterPro

Protein Sequence (498 amino acids)

>HMPREF1058_RS02700 glycerol kinase GlpK (Phocaeicola vulgatus CL09T03C04)
MISRIITIDQSTSATKAMLFSEDCELLHRVNIEHQQFYPQIGWVEHDAEEIYKNTIEAIH
CLLEQEEVNGKDISYSLAITNQRETVVVWNRHTGKSVYHAVVWQCQRGAAICKELKDKGY
SELVQRKTGLLIDPYFSASGAKWILDNVENARELAEKGDLLMGTIDSWLIWKLTEGRKHL
TDYTNASRTMLFNIHTLDWDEELLKLFTIPRSMMPEALPCDAVFGETTIEGLFKSPIQIA
GVLGDSHGALTGQMCFEAGMGKVTYGTGSSVMVNIGEEAVAAPEGLVTSVGFSALGKVYY
AFEGNIHCTGATIKWMVEKLGLVDSFNQIETLATSVKNNDGVYLVPAFTGLGAPWWKPDA
KAAIWGMTQNAGKAHVLRAGLESIAYQVKDLIDMMTRQAGIELKALRVDGGPTKNQFLMQ
FQADMLHAVINRSEIEEASALGAVVMNGFARKKWASFQEAAAMRTIDNCIAPCMEEKELQ
SLYSGWREAVKKVIGQNN